| 3株氟咯草酮降解菌株的筛选及其生物降解效果研究 |
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| 引用本文： | 雷向荣,沈硕,李玮. 3株氟咯草酮降解菌株的筛选及其生物降解效果研究[J]. 中国生物防治学报, 2021, 37(6): 1231-1240. DOI: 10.16409/j.cnki.2095-039x.2021.06.025 |
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| 作者姓名： | 雷向荣 沈硕 李玮 |
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| 作者单位： | 1. 青海大学, 西宁 810016;2. 青海省农林科学院, 西宁 810016;3. 青海省农业有害生物综合治理重点实验室, 西宁 810016;4. 青海省马铃薯育种重点实验室, 西宁 810016;5. 农业农村部西宁作物有害生物科学观测实验站, 西宁 810016 |
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| 基金项目： | 国家自然基金（31860512）；青海省应用基础研究项目（2018-ZJ-716） |
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| 摘 要： | 为探究土壤中具有降解氟咯草酮能力的菌株.采用富集法和平板稀释法筛选菌株,采用液相色谱-串联质谱法(LC-MS/MS)检测分析各菌株对氟咯草酮的降解率,结合形态学观察、16S rDNA和ITS测序分析对筛选得到的优势降解菌株进行鉴定,并采用室内生测法对各菌株进行安全性评价.结果表明,从土壤中获得3株优势降解菌株FL-M-...
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| 关 键 词： | 氟咯草酮 微生物降解 降解效果 安全性 |
| 收稿时间： | 2020-04-09 |
Screening of Three Strains for Degrading Flurochloridone and Their Biodegradation Effects |
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| LEI Xiangrong,SHEN Shuo,LI Wei. Screening of Three Strains for Degrading Flurochloridone and Their Biodegradation Effects[J]. Chinese Journal of Biological Control, 2021, 37(6): 1231-1240. DOI: 10.16409/j.cnki.2095-039x.2021.06.025 |
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| Authors: | LEI Xiangrong SHEN Shuo LI Wei |
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| Affiliation: | 1. Qinghai University, Xining 810016, China;2. Academy of Agriculture and Forestry, Qinghai University, Xining 810016, China;3. Key Laboratory of Agricultural Integrated Pest Management of Qinghai, Xining 810016, China;4. Key Laboratory of Potato Breeding of Qinghai Province, Xining 810016, China;5. Scientific Observing and Experimental Station of Crop Pest in Xining, Ministry of Agriculture and Rural Affairs, Xining 810016, China |
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| Abstract: | To explore the strains with the ability to degrade flurochloridone in the soil. Enrichment methods and plate dilution methods were used for screening strains, and the degradation rate of each strain for flurochloridone was detected by Liquid Chromatography-Tandem Mass Spectrometry. The strains with dominant degradation were identified by combined with morphological observations, 16S rDNA and ITS sequencing analysis, and the safety evaluation of each strain was determined with laboratory bioassays. The results showed that three strains FL-M-2, FL-M-3, FL-G-2 with a significant degradation rate from soil were identified as Purpureocillium lavendrulum, Didymellaceae sp. and Streptomyces parvus, respectively. Three strains cultured with 180 r/min, inoculation amount 5%, culture temperature at 28 ℃ for 7 days in culture medium have the degradation rate of 39.63%, 72.09% and 68.80% for the concentration of 1 mg/L flurochloridone, respectively. Among them, the strain FL-G-2 has the best degradation effect, and its degradation rate reached 84.37% after 14 days of culture. The fermentation broth of three strains had no obvious inhibitory effect on the growth of wheat and rape seedlings. This research provides a basis for analysis of the mechanism of microorganism degradation of flurochloridone, and also offers new strains resource for further development of the degradation of pyrrolidone herbicides. |
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| Keywords: | flurochloridone microbial degradation degradation effect safety |
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