| 赖氨酸去甲基化酶5C抑制剂CPI-455对ESCC细胞系 Eca-109增殖、凋亡的影响及其机制探讨 |
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| 引用本文： | 薛晓婕,' target='_blank'>,刘 彬,李文霞.赖氨酸去甲基化酶5C抑制剂CPI-455对ESCC细胞系 Eca-109增殖、凋亡的影响及其机制探讨[J].现代肿瘤医学,2021,0(13):2234-2238. |
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| 作者姓名： | 薛晓婕 ' target='_blank'> 刘 彬 李文霞 |
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| 作者单位： | 1.鄂东医疗集团黄石市中心医院（湖北理工学院附属医院）检验科，湖北 黄石 435000;
2.肾脏疾病发生与干预湖北省重点实验室，湖北 黄石 435000;
3.鄂东医疗集团黄石市中心医院（湖北理工学院附属医院），湖北 黄石 435000 |
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| 基金项目： | National Natural Science Foundation of China(No.81773018）；国家自然科学基金（编号:81773018）；湖北省卫生健康科研基金（编号:WJ2019H449)；湖北省医学青年拔尖人才计划 |
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| 摘 要： | 目的:本研究探讨赖氨酸去甲基化酶5C（lysine demethylase 5C，KDM5C）抑制剂CPI-455对食管鳞状细胞癌（esophageal squamous cell carcinoma，ESCC）细胞系Eca-109增殖、凋亡的影响及其机制。方法:运用MTT法、平板克隆形成实验检测 CPI-455对Eca-109细胞增殖的抑制作用；透射电镜观察CPI-455对Eca-109细胞线粒体内部超微结构的影响；流式细胞仪检测CPI-455诱导Eca-109细胞中活性氧（reactive oxygen species，ROS）水平；Western blot测定CPI-455对Eca-109细胞中p53、Bax、KDM5C、Cleaved Caspase-9和Cleaved Caspase-3蛋白表达水平的影响。结果:MTT法、平板克隆形成实验结果分析显示:CPI-455能够抑制Eca-109细胞的增殖，具有时间、浓度依赖性，差异有统计学意义（P均＜0.01）；电镜下观察Eca-109细胞内结构显示:CPI-455引起Eca-109细胞中的线粒体固缩，线粒体缩小，结构紧密；流式细胞术和Western blot显示:CPI-455可以上调Eca-109细胞中ROS含量、p53、Bax、Cleaved Caspase-9和Cleaved Caspase-3的蛋白表达，同时下调KDM5C蛋白表达（P＜0.05）。结论:CPI-455具有抑制Eca-109细胞增殖并诱导细胞凋亡的作用，其作用机制可能与下调KDM5C蛋白的表达有关。
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| 关 键 词： | KDM5C CPI-455 食管鳞状细胞癌 凋亡 Caspase |
The effect of lysine demethylase 5C inhibitor CPI-455 on proliferation and apoptosis in esophageal squamous cell carcinoma cell line Eca-109 and its mechanism |
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| XUE Xiaojie,' target='_blank'>,LIU Bin,LI Wenxia.The effect of lysine demethylase 5C inhibitor CPI-455 on proliferation and apoptosis in esophageal squamous cell carcinoma cell line Eca-109 and its mechanism[J].Journal of Modern Oncology,2021,0(13):2234-2238. |
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| Authors: | XUE Xiaojie ' target='_blank'> LIU Bin LI Wenxia |
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| Affiliation: | 1.Department of Clinical Laboratory，Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Hubei Huangshi 435000,China;2.Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention,Hubei Huangshi 435000,China;3.Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Hubei Huangshi 435000,China. |
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| Abstract: | Objective:To investigate the effect of lysine demethylase 5C inhibitor CPI-455 in esophageal squamous cells carcinoma（ESCC) Eca-109 cells of proliferation and apoptosis as well as its mechanism.Methods:MTT assay and plate colone formation assay were used to detect the inhibitory effect of CPI-455 on the proliferation of Eca-109 cells.Transmission electron microscopy was used to observe ultrastructural changes of esophageal cancer Eca-109 cell induced by CPI-455.Flow cytometry was used to detect the level of ROS in Eca-109 cells induced by CPI-455.The expressions of p53,Bax,KDM5C,Cleaved Caspase-9 and Cleaved Caspase-3 were detected by Western blot.Results:Analysis of the results of the MTT assay and plate clone formation assay showed that CPI-55 inhibited the proliferation of Eca-109 cells in a time and concentration dependent manner,the difference were statistical significance(P<0.01).The structure of Eca-109 cells under transmission electron microscopy showed that CPI-455 induced pyknosis of mitochondria in Eca-109 cells,reduced mitochondria and compacted structure.Flow cytometry and Western blot showed that CPI-455 can up-regulated the expression levels of ROS,p53,Bax,Cleaved Caspase-9 and Cleaved Caspase-3 in Eca-109 cells.The expression level of KDM5C were down-regulated(P<0.05).Conclusion:CPI-455 can inhibit the proliferation of ECA-109 cells and induce apoptosis,which may be related to down-regulation of KDM5C expression． |
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| Keywords: | KDM5C CPI-455 esophageal squamous cell carcinoma apoptosis Caspase |
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