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| 抑郁症模型鼠海马神经元细胞凋亡的初步研究 | |
| 引用本文： | 谢守付,马慧,刘伟,彭淼,丁宝坤.抑郁症模型鼠海马神经元细胞凋亡的初步研究[J].中国神经精神疾病杂志,2004,30(5):342-345. |
| 作者姓名： | 谢守付 马慧 刘伟 彭淼 丁宝坤 |
| 作者单位： | 1. 大连市第七人民医院,大连,116023 2. 燕山大学 3. 中国医科大学心理教研室 |
| 基金项目： | 本课题为国家自然科学基金资助项目(编号:30270497) |
| 摘 要： | 目的 研究抑郁症模型鼠海马神经元细胞的凋亡,探讨海马损害的病理生理机制。方法 选择旷场行为相近的成年SD雄性大鼠30只,随机分为实验组(15只)和对照组(15只),应用分养和长期不可预见的刺激制作成抑郁症模型。采用TUNEL染色法分析细胞凋亡,用免疫组化法检测凋亡的调控因子Bcl-xl。试验结果均采用SPSS 10.0软件处理。结果 与对照组比较,实验组大鼠海马内TUNEL染色阳性细胞增加,表达Bcl-xl蛋白的阳性细胞减少,两组有显著性差异。结论 抑郁症模型鼠海马神经元细胞凋亡增加。 |
| 关 键 词： | 慢性应激 海马 凋亡 抑郁症 |
| 修稿时间： | 2004年4月12日 |
studying apoptosis in the hippocampus of the depression model of rats |
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| Xie Shoufu,Ma Hui,Liu Wei,Pen Miao,Ding Baokun.studying apoptosis in the hippocampus of the depression model of rats[J].Chinese Journal of Nervous and Mental Diseases,2004,30(5):342-345. | |
| Authors: | Xie Shoufu Ma Hui Liu Wei Pen Miao Ding Baokun |
| Affiliation: | Xie Shoufu,Ma Hui,Liu Wei,Pen Miao,Ding Baokun. The Dalian Seventh People Hospital,179 Lingshui street,Dalian. 116023. Tel:0411-84671212-309 |
| Abstract: | Objective To study the pathophysiological mechanism about the apoptosis of neurons in the rat hippocampus of depression model. Methods The depression model of rat was produced by separation and chronic unpredictable mild stress. The apoptosis was detected by techniques of terminal deoxynucleotibyl transferase(TdT)-mediated dUTP nick end labling(TUNEL) . Im-munohistochemistry method was used to measure the expression of Bcl-xl in the hippocampus. Statistical analysis was carried out with SPSS 10.0. Results The percent age of positive cells in the hippocampus of stress group increased compared with control group in the TUISEL method ( P < 0.01) . In the immunohistochemistry method, the result was just reverse. Conclusions The apoptosis of neurons of hippocampus increased in the rat model of depression. |
| Keywords: | Chronic stress Hippocampus Apoptosis Depression |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
