罗非鱼不同组织脂肪酸含量的分析

分别取罗非鱼(Oreochromis niloticus)的肉、脂肪、肝、眼和皮等组织,用氯仿-甲醇(2∶1,v/v)提取,以C17∶0为内标物,利用气相色谱-质谱法(GC/MS)分析了各组织脂肪酸种类及含量。结果显示:罗非鱼各种组织中含有16种脂肪酸,富含多不饱和脂肪酸。脂肪组织中的脂肪酸含量最高,其次是眼睛、肝脏、鱼肉和鱼皮;各组织中饱和脂肪酸(SFA)含量为202~8869 mg/kg,主要为棕榈酸(C16∶0)和硬脂酸(C18∶0);单不饱和脂肪酸(MUFA)含量为293~11219 mg/kg,主要为棕榈油酸(C16∶1)和油酸(C18∶1);多不饱和脂肪酸(PUFA)含量为362~8293 mg/kg,主要是亚油酸(C18∶2)、亚麻酸(C18∶3)和二十二碳六烯酸(DHA,C22∶6);n-6/n-3为1.20~2.46。

The content of fatty acids in different tissue of tilapia(Oreochromis niloticus)

With the C17∶0 as internal standard,the fatty acids in tilapia(Oreochromis niloticus) fish fresh,skin,fats,eyes and liver were extracted by Chloroform-methanol(2∶1) and analyzed by gas chromatography/mass spectrometry(GC/MS).The results showed that tilapia had 16 kinds of fatty acid,and was rich in unsaturated fatty acids;fatty acid content in the fat tissue was highest,followed by the eyes,liver,fish flesh and skin.In different tissues of Tilapia,the saturated fatty acids(SFA) content was 202~8869 mg/kg,mainly palmitic acid(C16∶0) and stearic acid(C18∶0);the monounsaturated fatty acids(MUFA) content was 293~11219 mg/kg,mainly palmitoleic acid(C16∶1) and oleic acid(C18∶1);and the polyunsaturated fatty acids(PUFA) content was 362~8293 mg/kg,mainly linoleic acid(C18∶2),linolenic acid(C18∶3) and docosahexaenoic acid(DHA,C22∶6),and n-6/n-3 fatty acids ratio was 1.20~2.46.