Protein kinase C, a coupling element between stimulus and secretion of basophils |
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Authors: | R Sagi-Eisenberg I Pecht |
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Affiliation: | Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel 76100 |
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Abstract: | Protein kinase C plays a crucial role in the transmission and control of secretory cell membranal signals. This Ca2+ and phospholipid dependent kinase have been isolated and partially purified from histamine secreting rat basophilic leukemia cells (RBL-2H3 line). The tumor promoter 12-O-tetradecanoyl phorbol-13-acetate ester (TPA) directly activated this isolated enzyme. In the intact RBL-2H3 cells, TPA did not significantly affect free intracellular Ca2+ ions concentration or induce secretion. However, at low concentrations it synergistically enhanced secretion induced either by antigen or ionophore. Significantly, at TPA concentrations exceeding 25 ng/ml both the increase in cytosolic free Ca2+ and the ensuing degranulation were inhibited. The synergism between TPA and the ionophore reaches saturation. These findings suggest that free cytosolic Ca2+ and kinase C-mediated protein phosphorylation are synergistically involved in the mediation of the cellular response. Moreover, kinase C appears to play a dual role both in the activation and termination of secretion. The latter is most probably achieved by closure of the Ca2+ channels in the cells. |
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Keywords: | protein kinase C basophils secretory cells |
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