HIF-1与VEGF在翼状胬肉中的表达及意义

目的研究缺氧诱导因子-1（HIF-1）与血管内皮生长因子（VEGF）在翼状胬肉组织中的表达及其相关性。方法采用免疫组织化学法研究HIF-1与VEGF分别在32例翼状胬肉与11例人正常球结膜组织中的表达。结果32例翼状胬肉中HIF-1的阳性表达率为62.5%（20/32）,VEGF的阳性表达率为84.8%（27/32）,11例正常结膜组织中HIF-1的阳性表达率为9.1%（1/11）,VEGF的阳性表达率为18.2%（2/11）,正常球结膜与翼状胬肉组织中HIF-1与VEGF的表达差异有统计学意义,且翼状胬肉中HIF-1与VEGF的表达呈正相关（P〈0.05）。结论HIF-1及VEGF在翼状胬肉中高表达,提示其可能参与了翼状胬肉的发生和发展。HIF-1与VEGF在翼状胬肉中的表达呈正相关,提示在翼状胬肉中HIF-1可能参与了对VEGF的调控。

Expression and significance of HIF-1 and VEGF in pterygium

Objective It has been well-known that vascular endothelial growth factor（VEGF）is an important stimulating factor in pathogenesis of neovascularization and a target factor of hypoxia inducible factor-1（HIF-1）.So HIF-1 is a main regulating factor of tumor neovascular formation.This study was to investigate the effect and relationship between HIF-1 and VEGF in pathogenesis of pterygium.MethodsThe 32 pterygium specimen was obtained during the operation and the 11 normal conjunctival specimen was collected during cataract extraction and strabismus correcting surgery.The mass pathological examination was performed under the optical microscope.The expression of HIF-1 and VEGF in pterygium and normal conjunctiva was examined using immunohistochemistry and the positive rate was calculated by the scoring strain intensity and numbers of positive cells.ResultsThe squamous metaplasia,a lots of inflammatory cells,fibroblasts and new blood vessels were seen under the optical microscope in pterygium specimen.The positive rate of HIF-1 was 62.5% in pterygium（score：8.90±0.86）and was 9.1% in normal conjunctiva（score：4.30±0.79）,and the positive rate of VEGF was 84.8% in pterygium（score：9.90±0.89）and 18.2% in normal conjunctiva（score：4.80±0.87）,showing significant differences in the expressions of HIF-1 and VEGF between pterygium and normal conjunctiva.The expression of HIF-1 was positively correlated with that of VEGF in pterygium（r=0.389,P=0.037）.ConclusionThe high expression of HIF-1 and VEGF in pterygium suggests that HIF-1 and VEGF may play an important role in the development of pterygium.The expression of HIF-1 is positively correlated with VEGF in pterygium,which indicate that HIF-1 might participate in the regulation of VEGF.