外周血淋巴细胞LFA-1水平与肝移植急性排斥的关系

【目的】探讨大鼠原位肝脏移植(OLTx)后外周血淋巴细胞表面淋巴细胞功能相关抗原-1（LFA-1）与急性排斥的关系。【方法】实验动物分为两组，非排斥对照组供体、受体均为SD大鼠，各20只，排斥组供体为Wistar大鼠，受体为SD大鼠，各20只。受体大鼠分别于原位肝移植术前1天及术后第1、3、5、7天从尾静脉采血，经免疫荧光抗体-流式细胞术检测淋巴细胞表面LFA-1的表达。【结果】原位肝移植术后，受体大鼠外周血淋巴细胞LFA-1呈低水平表达，与术前相比差异具显著性意义（P<0.01）;受体大鼠发生急性排斥反应时，外周血淋巴细胞LFA-1水平显著增高，与对照组相比差异具显著性意义（P<0.01）。【结论】检测外周血淋巴细胞LFA-1表达水平有助于移植肝脏急性排斥的诊断。

Relationship Between the Expression of Lymphocyte Function-associated Antigen-1 on Peripheral Lymphocytes and Acute Rejection After Liver Transplantation

To investigate the relationship between the expression of lymphocyte function associated antigen 1 (LFA 1) on peripheral lymphocytes and acute rejection after orthotopic liver transplantation (OLTx) in rat. Adult male rats were divided into 2 groups. In the non rejection group, 40 SD rats were used as both donors and recipients. In the rejection group, 20 Wistar rats were used as donors and 20 SD rats as recipients. Blood samples were collected through the tail vein 1 day before transplantation and on days 1?3?5?7 after OLTx. The expression of LFA 1 (CD11a) on peripheral lymphocytes was analyzed by using indirect immunofluorescent marker flow cytometry. The expression level of LFA 1 on peripheral lymphocytes in recipient rats after orthotopic liver transplantation was markedly lower than that before operation (P<0 01); The expression level of LFA 1 on peripheral lymphocytes in rats with acute liver rejection was significantly higher than that in the non rejection group (P<0 01).Conclusion] Monitoring the expression level of LFA 1 on peripheral lymphocytes may be helpful to the diagnosis of acute graft rejection.