部分耐冬山茶栽培品种的AFLP分析

 【目的】从分子水平分析耐冬山茶品种间的遗传关系,探讨其品种分类问题。【方法】利用荧光AFLP技术,采用8对EcoRⅠ/Mse 1引物对耐冬山茶16个品种及2个变异类型、5个山茶南方品种、2个云南山茶品种进行AFLP分析,得到清晰的相关AFLP谱带。【结果】在所测试的30个样品基因组中,8对AFLP引物共获得1 062条清晰的谱带,记录了987条多态性带,多态性带占总带数的92.9%,按照非加权算术平均数聚类法(UPGMA)构建树状分支图。【结论】耐冬山茶与南方山茶的亲缘关系较近;可将耐冬山茶品种分成6个亲缘关系不同的组,表明耐冬山茶有丰富的遗传变异。

Study on the Genetic Diversity of Some Camellia japonica (Naidong) Cultivars Based on AFLP Markers

【Objective】The genetic diversity of Camellia Japonica (Naidong) cultivar accessions was analyzed at molecular level,and the helpful information for cultivar classification was obtained.【Method】The genetic diversity of 16 cultivars and 2 mutation types of Camellia Japonica (Naidong) with 5 cultivars of C. japonica from south of China, 2 cultivars of C. reticulata was analyzed by the fluorescent-AFLP marker techniques with 8 pairs of EcoRⅠ/Mse 1 primer combinations. 【Result】 In the genomes of the 30 tested samples,a total of 1 062 spectral bands amplified from the 8 pairs of primer combinations were selected from AFLP analysis and 987 bands appeared to be polymorphic (92.9%). The data were used to calculate the Nei’s genetic distance and to construct a dendrogram based on UPGMA cluster analysis. 【Conclusion】 It showed that the cultivars of Camellia japonica (Naidong) have a remote genetic relationship compared with the cultivars of Camellia from south of China,and cultivars of Camellia japonica (Naidong) can be divided into 6 groups,which indicated that Camellia japonica (Naidong) have rich genetic variability.