某氡温泉周边居民外周血血浆中miRNAs的表达

目的 探讨河北省某地氡温泉对周围居民外周血血浆中miR-16、miR-106b、miR-449a、miR-34a和let-7g表达的影响。方法 采用简单随机抽样法,抽取某地氡温泉周边居民41人;同时简单随机抽取生活习惯相似,但未长期接触过氡温泉的居民46人。采用实时荧光定量PCR(qRT-PCR)方法定量检测所有受检者血浆miRNAs的表达水平。结果 在氡温泉组血浆中,miR-16、miR-106b、miR-449a和let-7g的表达显著高于健康对照组(Z=-2.278、-3.835、-2.719、-2.721,P<0.05)。miR-16、miR-106b、miR-449a和let-7g的表达改变与氡暴露因素有关(t=2.154、3.711、2.319、2.015,P<0.05),而与年龄、性别、吸烟和饮酒等因素无关。miR-34a在氡温泉组和健康对照组血浆中的表达差异无统计学意义。结论 miR-16、miR-106b、miR-449a和let-7g可作为识别氡暴露的潜在生物学指标。

Expression of miRNAs in peripheral blood plasma of the residents surrounding hot springs with radon

Objective To investigate the expressions of miR-16, miR-106b, miR-449a, miR-34a and let-7g in peripheral blood plasma of the residents surrounding hot springs with radon in Hebei province.Methods A total of 41 randomly selected residents surrounding hot springs with radon were considered as the radon group, and 46 residents with same living habit but without contact with hot springs were considered as control. The miRNAs in the peripheral blood plasma of these two groups were detected with qRT-PCR.Results The levels of miR-16, miR-106b, miR-449a and let-7g in the radon group were significantly higher than those in control group (Z=-2.278, -3.835, -2.719, -2.721,P<0.05). Alterations of these miRNAs were associated with radon exposure (t=2.154, 3.711, 2.319, 2.015,P<0.05) but had no relationship with age, sex, smoking and drinking factors. No significant difference was observed in the plasma levels of miR-34a between the two groups.Conclusions miR-16, miR-106b, miR-449a and let-7g could be applied as potential biomarkers for radon exposure.