| Pt(Ⅱ)与甲胎蛋白的相互作用机理研究及其分析应用 |
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| 引用本文： | 干宁,侯琳熙,王鲁雁,李天华,徐伟民.Pt(Ⅱ)与甲胎蛋白的相互作用机理研究及其分析应用[J].贵金属,2007,28(3):36-39. |
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| 作者姓名： | 干宁 侯琳熙 王鲁雁 李天华 徐伟民 |
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| 作者单位： | 宁波大学,材料与化工学院,宁波市新型功能材料及其制备科学国家重点实验室,浙江,宁波,315211 |
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| 基金项目： | 国家自然科学基金
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浙江省科技攻关项目 |
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| 摘 要： | 采用电化学和荧光方法研究了Pt(Ⅱ) 水合物与甲胎蛋白(AFP)相互作用的机理,发现它们具有较强的相互结合能力.文中对Pt(Ⅱ)- AFP体系的适宜反应条件、影响因素及信号强度与AFP浓度的关系进行了研究.结果表明,在一定Pt(Ⅱ)浓度范围内,AFP浓度与其荧光峰电流强度成正比.本方法有较高的灵敏度,对AFP的检出限为10.2ng/mL.考察了共存物质的干扰影响,并对实际血清样品中的AFP进行分析,结果满意.
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| 关 键 词： | 分析化学 电化学 荧光 甲胎蛋白 Pt(Ⅱ) |
| 文章编号： | 1004-0676（2007）03-0036-04 |
| 修稿时间： | 2006-10-31 |
Study on Interaction between Platinum and Alpha Fetoprotein and Relative Analytical Application |
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| GAN Ning,HOU Linxi,WANG Luyan,LI Tianhua,XU Weimin.Study on Interaction between Platinum and Alpha Fetoprotein and Relative Analytical Application[J].Precious Metals,2007,28(3):36-39. |
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| Authors: | GAN Ning HOU Linxi WANG Luyan LI Tianhua XU Weimin |
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| Affiliation: | The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ninbo University, Zhejiang, Ninbo 315211, China |
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| Abstract: | The reaction of aqueous platinum(II) hydrate with alpha fetoprotein proteins(AFP) was studied by electrochemical and fluoremetry method.The results show that Pt(II) can react with AFP strongly.Therefore,a novel method for the determination of trace amounts of AFP by fluoremetry method has been established.In the certain range of Pt(II),the concentration of AFP is directly proportional to the enhanced intensity of FL.The suitable reaction conditions,affecting factors as well as the influence of some coexisting substances were investigated.The method has high sensitivity,with the detection limit of 10.2ng/mL for AFP protein and good selectivity.It was applied to the determination of AFP in practical samples with satisfactory results. |
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| Keywords: | analytic chemistry electrochemistry fluoremetry serum protein Pt(II) |
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