两种不同电针刺激强度和频率对大鼠脑内单胺类神经介质影响的比较

<正> 目前针刺临床和针刺原理研究中常常采用电针代替手法捻针,因此,电针刺激参数成为针刺临床和针刺原理研究中的一个基本重要研究问题。这方面的研究对阐明针刺原理和提高针刺治疗效果将是非常必要的。电针刺激参数包括波形、波幅、波宽、频率、节律和持续时间等。本文比较了两种不同电针刺激强度和频率对大鼠不同脑区内5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、去甲肾上腺素(NA)和多巴胺(DA)水平的影响。

COMPARISONS OF EFFECTS OF ELECTROACUPUNCTURE STIMULATIONS OF TWO DIFFERENT INTENSITIES AND FREQUENCES ON MONOAMINE NEUROTRANSMITTERS IN RAT BRAIN REGIONS

Effects of electroacupuncture stimulations of two different intensities (pulse amplitude 3V, 6V)and frequencies(10Hz, 200Hz)on levels of 5-HT, HIAA, NA and DA were compared in 6 rat brain regions (oblongata plus pons, hypothalamus, hippocampus, striatum, midbrain and cortex). 1.5-HT levels in the oblongata plus pons and cortex were increased by electroacupuncture stimulation of 6V, but were not increased by 3V. The frequency of 10Hz was no obvious difference as compared with 200Hz in the effects on brain 5-HT concentrations. 2.5-HIAA concentrations in the striatum and cortex were increased only using electroacupuncture stimulations of combination of higher intens- ity (6V) and lower frequency (10Hz). 3. NA level in the striatum was decreased by frepuency of 200 Hz, but was not decreased by 10 Hz, the effect of 3V on striatum NA level was no significant different as compared with 6V.Responses of NA concen- tration in the cortex to electroacupuncture stimulations of different intensi- ties and frequencies seemd to be different as compared with that in the stri- atum 4. Both frequency and intensity parameters of electroacupuncture were involved in the increases of DA levels in brain, but proper combinations of differet pulse amplitudes with different frequencies were necessary for the DA increases in brain. In addition, the response of DA level in the oblon- gata plus pons to different electroacupuncture parameters also were differ- ent from that in the hypothalamus. The results partially explain contradictions of the results obtained by laboratories using various electroacupuncture parameters in studies on rela- tionships between acupuncture and monoamine neurotransmitters. Therefore, the studies on the parameters would be very important for deep-going rese- arch of acupuncture mechanism and raising acupuncture clinical efficiency.