| 机会性筛查人群中高危型人乳头瘤病毒与宫颈病变相关性的分析 |
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| 引用本文： | 边立华,李颖,王晓莉,吴晓舟,宋磊,刘宏图.机会性筛查人群中高危型人乳头瘤病毒与宫颈病变相关性的分析[J].中华实验和临床病毒学杂志,2011,25(1):89-91. |
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| 作者姓名： | 边立华 李颖 王晓莉 吴晓舟 宋磊 刘宏图 |
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| 作者单位： | 中国人民解放军总医院妇产科,北京,100853;中国疾病预防控制中心病毒病预防控制所,病毒相关肿瘤室病毒基因工程国家重点实验室;北京大学人民医院中心手术室; |
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| 基金项目： | 艾滋病和病毒性肝炎等重大传染病防治专项 |
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| 摘 要： | 目的 研究第二代杂交捕获法(HC2)高危型人乳头瘤病毒(HR-HPV)检测阳性样品中HPV与宫颈病变的相关性.方法 随机抽取HC2阳性患者602名(344名行细胞学检查),采用PCR-反向点杂交(RDB)法对HPV分型并分析HPV型别与细胞学的相关性.结果 602例患者PCR阳性率94.5%;HPV分型检出率依次为HPV16、52、58、56、39等;HC2检测谱外单独感染24例;HPV16、HPV16/18与ASCUS、LSIL、HSIL有明显相关性.结论 机会性筛查人群中最常见HPV型别依次为HPV16、52、58;HC2检测的假阳性率为4.0%;HPV16与宫颈病变程度相关.
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| 关 键 词： | 杂交 乳头状瘤状病毒 人 基因分型 细胞学技术 |
Study for association between high risk human papillomavirus and cervical lesions in the samples from opportunistic screening |
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| BIAN Li-hua,LI Ying,WANG Xiao-li,WU Xiao-zhou,SONG Lei,LIU Hong-tu.Study for association between high risk human papillomavirus and cervical lesions in the samples from opportunistic screening[J].Chinese Journal of Experimental and Clinical Virology,2011,25(1):89-91. |
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| Authors: | BIAN Li-hua LI Ying WANG Xiao-li WU Xiao-zhou SONG Lei LIU Hong-tu |
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| Abstract: | Objective To investigate the association between HPV genotypes and cervical lesion in Hybrid Capture 2 ( HC2 ) HPV test positive samples. Methods 602 cervical samples randomly obtained detected as HPV positive by the HC2 high-risk probe cocktail were determined by polymerase chain reaction (PCR)-reverse dot blot (RDB), among them 344 participated Thinprep Cytology test. Results 569 (94. 5% ) samples were successfully amplified. The most common HPV genotypes were HPV16(31.6% ),52 ( 16. 7 % ), 58 ( 15.1% ), 56 ( 8. 1% ), 39 ( 7. 9% ) ; HPV 16 or HPV 16/18 infection was significantly related to ASCUS, LSIL and HSIL; 24 samples suggested single HPV infection with the genotypes not available for HC2. Conclusion The most common HPV genotypes in random screening were HPVI6, 52 and HPV58; Our data demonstrated the pseudo-positivity of HC2 test was 4.0% ; HPV16 was a index for progression of HSIL. |
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| Keywords: | HybridizationPapillomavirus humanGenotypingCytological techniques |
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