| 药根碱与牛血清白蛋白相互作用的光谱研究 |
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| 引用本文： | 李建晴,刘毓芳,蔡雪梅,卫艳丽,董川.药根碱与牛血清白蛋白相互作用的光谱研究[J].化学试剂,2011,33(6):486-488,496. |
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| 作者姓名： | 李建晴 刘毓芳 蔡雪梅 卫艳丽 董川 |
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| 作者单位： | 1. 晋中学院,化学化工学院,山西晋中,030600
2. 山西大学,环境科学与工程研究中心,山西太原,030006 |
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| 基金项目： | 国家自然科学基金资助项目(20875059); 山西省归国留学基金资助项目(2008); 山西省高校科技研究开发项目(20091036) |
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| 摘 要： | 采用荧光和UV光谱法研究了盐酸药根碱(Jat)与牛血清白蛋白(BSA)相互作用的光谱特性.结果表明:Jat对于BSA荧光猝灭主要是静态猝灭和非辐射能量转移;Jat浓度增大,BSA荧光峰被猝灭的同时出现峰裂分现象,原来345 nm处的单峰逐渐裂分为二重峰,其中一峰峰值蓝移,另一峰峰值红移至λcm=362～365 nm;测...
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| 关 键 词： | 盐酸药根碱 牛血清白蛋白 荧光光谱法 |
Interaction between jatrorrhizine and bovine serum albumin |
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| LI Jian-qing,LIU Yu-fang,CAI Xue-mei,WEI Yan-li,DONG Chuan.Interaction between jatrorrhizine and bovine serum albumin[J].Chemical Reagents,2011,33(6):486-488,496. |
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| Authors: | LI Jian-qing LIU Yu-fang CAI Xue-mei WEI Yan-li DONG Chuan |
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| Affiliation: | LI Jian-qing1,LIU Yu-fang1,CAI Xue-mei1,WEI Yan-li2,DONG Chuan2 (1.Department of Chemistry and Chemical Engineering,Jinzhong College,Jinzhong 030600,China,2.Environmental-science and Engineering-study center,Shanxi University,Taiyuan 030006,China) |
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| Abstract: | The interactions between jatrorrhizine(Jat) and bovine serum albumin(BSA) were studied by fluorescence and ultraviolet-visible absorption spectra.The binding constants and binging sites were measured at different temperatures.The results revealed that Jat have strong ability to quench the intrinsic fluorescence of BSA.The phenomenon that fluorescence spectra of BSA splitted and shifted from a simple peak to double peaks in the presence of Jat concentration accretion.Fluorescence quenching was thought to be ... |
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| Keywords: | jatrorrhizine bovine serum albumin fluorescence |
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