竹黄菌液态发酵产漆酶培养条件的优化

分离筛选到一株产漆酶的竹黄菌,采用单因子相互比较法,研究了该菌株的最适发酵产酶条件。确定了最适的碳源为2 g/dL可溶性淀粉、最适的氮源为0.8 g/dL的酵母膏,以及最适铜离子浓度为2.4 mmol/L,在自然pH 5.0,装液量为50 mL,30℃、200 r/min摇瓶振荡培养64 h下,产漆酶酶活水平达120 000 U/L,比优化前提高近17倍。该竹黄菌株与已报道的白腐真菌相比,具有发酵周期短且产漆酶酶活较高的优点。

Optimization of Shiraia bambusicola Liquid-State Fermentation for Laccase Production

A new laccase producing strain,Shiraia bambusicola,was isolated from the natural screening.In this study,the optimum nutrition and environmental conditions were investigated and listed as follows: f soluble starch 2%,yeast extract 0.8%,and copper ion 2.4 mmol/L,pH 5.0,liquid volume 50/250 mL,30 ℃,200 r/min.With the optimum conditions,the activity of laccase was achieved at 120 000 U/L after 64 h,was higher 17 times than that of the control.Compared with the reported white-rot,Shiraia bambusicola has the advantages of short fermentation period and higher laccase activity.