UPLC-Q-TOF-MS测定治咳川贝枇杷滴丸中6种有效成分的含量

 目的 建立同时测定治咳川贝枇杷滴丸中6种有效成分（鸟苷、贝母素乙、桔梗皂苷D、桔梗皂苷D₃、齐墩果酸和熊果酸）含量的超高压液相色谱串联四级杆飞行时间质谱联用（UPLC-Q-TOF-MS）的检测方法。方法 采用Waters Acquity BEH C₁₈色谱柱（2.1 mm×100 mm，1.7 μm)，流动相为乙腈-甲酸-水体系梯度洗脱，流速为0.4 mL·min^-1，PDA检测190~400 nm扫描；进样量：2.0 μL。结果 6种成分在一定范围内均呈现良好线性，r均大于0.999 6；精密度、重复性和稳定性良好；加样回收率在99%~101%之间。 结论 所建立的方法简便、准确、快速、重复性好，可用于治咳川贝枇杷滴丸中鸟苷、贝母素乙、桔梗皂苷D、桔梗皂苷D₃、齐墩果酸和熊果酸6个有效成分的含量测定。

Determination of Six Active Components in Zhikechuanbeipipa Dropping Pills by UPLC-Q-TOF

OBJECTIVE To develop a UPLC-Q-TOF-MS method for determination of six components(guanosine,peiminine,platycodin D,platycodin D3,oleanolic acid and ursolic acid) in Zhikechuanbeipipa dropping pills.METHODS The analysis was performed on a Waters Acquity BEH C18 column(2.1 mm×100 mm,1.7 μm)with the mixture of acetonitrile-water-formic acid as mobile phase,and the flow rate was 0.4 mL· min-1;The PDA detection wavelength was 190-400 nm;The column temperature was room temperature(30 ℃).RESULTS The linear relationship between the concentration and peak areas of the six compounds were all linear(r>0.999 6).The average recoveries(n=6) were between 99%-101%.CONCLUSION This method was simple,accurate and reliable to determine the six contents of Zhikechuanbeipipa dropping pills for the quality control.