| 下调HMGA2基因表达对改善骨肉瘤U2OS细胞恶性表型的实验研究 |
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| 引用本文： | 曲珊珊,李荣贵,张海英,王洋,史艳芬,吕慧,李玉林.下调HMGA2基因表达对改善骨肉瘤U2OS细胞恶性表型的实验研究[J].中国免疫学杂志,2010,26(3). |
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| 作者姓名： | 曲珊珊 李荣贵 张海英 王洋 史艳芬 吕慧 李玉林 |
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| 作者单位： | 吉林大学白求恩医学院,病理生物学教育部重点实验室,长春,130021 |
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| 摘 要： | 目的:研究HMGA2表达在维持人骨肉瘤U2OS细胞恶性表型中的作用,为基因靶向治疗提供理论依据。方法:采用基于DNA的shRNA表达载体HMGA2-shRNA,稳定转染人骨肉瘤U2OS细胞,下调其HMGA2表达水平,并应用RT-PCR技术检测其对HMGA2基因的沉默效果;经Cell Counting Kit-8(CCK8)测定、Hoechst33342染色荧光显微镜观察及Boyden小室法分别检测细胞增殖、凋亡及迁移情况;实时定量RT-PCR检测mRNAs表达水平。结果:稳定转染靶向HMGA2的shRNA可特异性下调U2OS细胞HMGA2 mRNA表达水平;其作用结果使细胞的增殖和迁移能力受到明显抑制,自发凋亡率及Caspase 3和Caspas 9基因表达水平显著升高。结论:HMGA2基因异常表达在维持人骨肉瘤U2OS细胞恶性表型中起重要作用,靶向HM-GA2的基因治疗可能为骨肉瘤治疗带来新希望。
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| 关 键 词： | HMGA2基因 骨肉瘤U2OS 细胞增殖 细胞凋亡 |
Down regulation of HMGA2 expression changes malignant phenotypes the malignant phenotype of human osteosarcoma U2OS cells |
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| QU Shan-Shan,LI Rong-Gui,ZHANG Hai-Ying,WANG Yang,SHI Yan-Fen,L Hui,LI Yu-Lin.Down regulation of HMGA2 expression changes malignant phenotypes the malignant phenotype of human osteosarcoma U2OS cells[J].Chinese Journal of Immunology,2010,26(3). |
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| Authors: | QU Shan-Shan LI Rong-Gui ZHANG Hai-Ying WANG Yang SHI Yan-Fen L Hui LI Yu-Lin |
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| Affiliation: | QU Shan-Shan,LI Rong-Gui,ZHANG Hai-Ying,WANG Yang,SHI Yan-Fen,L(U) Hui,LI Yu-Lin |
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| Abstract: | Objective:The roles of HMGA2 in maintaining malignant phenotypes of the osteosarcoma U2OS cells was studied to explore the possibilities for it to be developed as a target for gene therapy.Methods:U2OS cells were stably transfected with a DNA based shRNA expression vector which targeted to HMGA2.The expression of HMGA2 mRNA was proved by RT-PCR;Cell growth,migration and apoptosis were determined with CCK8,hoechst33342 staining and Boyden ventricle,respectively.The mRNA levels of Caspase 3 and Caspase 9 were... |
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| Keywords: | shRNA HMGA 2 shRNA U20S Proliferation Apoptosics |
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