急性胰腺炎大鼠血浆C肽、胰岛素、胰高糖素的变化及其意义

目的观察急性胰腺炎大鼠血中C肽(CP)、胰岛素(INS)、胰高糖素(GG)和血糖水平,以揭示胰岛内分泌细胞的功能变化。方法将120只大鼠随机平均分为A组(急性胰腺炎)和B组(假手术对照组),每组60只。用放射免疫分析法测定大鼠血中CP,INS和GG水平,同时测定血糖值。大鼠剖腹后肉眼观察胰腺形态学变化,取胰腺标本行组织病理学检查。结果制模9h后病理检查示A组大鼠均表现为重型急性胰腺炎。A组CP于术后9h,INS于术后15h和GG于术后12h开始明显高于对照组(P<0.01,P<0.05,P<0.05)。结论(1)急性胰腺炎时大鼠血中CP和INS水平可反映胰岛B细胞功能受损;CP能更准确、更及时地反映胰岛B细胞受损程度,并与病理学变化同步。(2)GG水平可反映胰岛A细胞功能;急性胰腺炎时A细胞可能比B细胞更易于受到破坏。

Significance of changes of C peptide, insulin and glucagon levels in serum of rats with acute pancreatitis

Objective To investigate the levels of C peptide,insulin,glucagon and glucose and the function of islet endocrine cells in rats with acute pancreatitis.Methods one hundred and tweenty rats were randomly divided to two groups:Group A(acute pancreatitis,AP)and Group B(sham operation,SO).Blood C peptide,insulin,glucagon and glucose were determined.The pancreatic morphology was observed after laparotomy.Pancreas tissue was excised to be observed in terms of histopathology.Results The levels of C peptide,insulin and glucagon in group A were significantly higher than those in the group B at 9h,15h and 12h after the procedure respectively(P<0.01,P<0.05 and P<0.05,respectively).Histopathologjcally,the rats in group A had severe acute pancreatitis at 9h after the procedure.Conclusions(1)The islet endocrine cells function of rats with acute pancreatitis is impaired,the levels of C-peptide and insulin in serum can reflect the function of islet B cells.C-peptide can more accurately and more quickly reflect the degree of B cells injury,and its changes are synchronous with the histopathological changes in B cells.(2)The level of glucagon can reflect the function of pancreas A cells.The islet A cells may be more susceptible than B cells to be damaged in acute pancreatitis.