| HPLC法测定清肺消炎丸中盐酸麻黄碱的含量 |
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| 引用本文： | 罗辉,王原,李昂,闫晓楠.HPLC法测定清肺消炎丸中盐酸麻黄碱的含量[J].世界科学技术,2009,11(3):461-464. |
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| 作者姓名： | 罗辉 王原 李昂 闫晓楠 |
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| 作者单位： | 罗辉,李昂(天津中新药业集团股份有限公司达仁堂制药厂,天津,300457);王原,闫晓楠(天津中新药业研究中心,天津,300457) |
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| 摘 要： | 目的：建立HPLC法测定清肺消炎丸中盐酸麻黄碱含量的方法。方法：色谱柱：Phenomenex GEMINI C18（5μm，4．6mm×250mm）；流动相：乙腈-0．02mol·L^-1磷酸二氢钾溶液（磷酸调pH值至2．7）（4：96）；检测波长：207nm；柱温：30℃；流速：1．0mL·min^-1。结果：线性范围为0．0416～0．6656μg（r=0．9999），平均回收率为99．71％，RSD为0．75％，重复性RSD为1．12％（n=6），精密度RSD为1．27％（n=6），稳定性RSD为0．80％。结论：本文所得方法稳定、可靠，可作为该制剂的质量控制方法。
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| 关 键 词： | 清肺消炎丸 盐酸麻黄碱 HPLC |
HPLC Determination of Ephedrine Hydrochloride Content in Qingfei Xiaoyan Pill |
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| Affiliation: | Luo Hui, Wang Yuan, Li Ang, Yan Xiaonan (1.Darentang Pharmacy, Zhongxin Pharmaceuticals, Tianjin 300457, China; 2. R＆D Center, Zhongxin Pharmaceuticals, Tianjin 300457, China) |
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| Abstract: | Authors established an HPLC method to determine Ephedrine hydrochloride content in Qingfei Xiaoyan Pill. In the study, Ephedrine hydrochloridein were assayed on a Phenomenex GEMINI C18（5μm, 4.6mm×250mm）column, with the mobile phase composed of Acetonitrile-0.02mol·L^-1 KH2PO3 solution （adjusted to PH=2.7 with phosphoric acid） （4： 96） at a flow rate of 1.0 ml·min^-1. The determination wavelength was defined at 207 nm. Results show linear calibration curves were ranged from 0.0416μg to 0.6656μg （r = 0.9999）for Ephedrine hydrochloridein. The averaged recovery （n=6） of Ephedrine hydrochloridein was 99.71% （RSD=0.75%）. The method has been proved simple, reliable, accurate, and repeatable, and is effective for the quality control of Qingfei Xiaoyan Pill. |
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| Keywords: | HPLC |
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