高效液相色谱法测定保健食品中丙氨酰-酪氨酸的含量

目的 建立高效液相色谱法测定保健食品中丙氨酰-酪氨酸含量的分析方法。方法 样品经一级水超声提取, 采用Agilent C18柱(150 mm×4.6 mm, 2.7 μm)进行分离, 以乙腈-水溶液为流动相进行等度洗脱, 采用高效液相色谱法检测, 外标法定量。结果 很好地分离了丙氨酰-酪氨酸, 在浓度0.01~0.05 mg/mL之间呈现良好的线性关系, 平均回收率在95%~105%之间, 相对标准偏差(relative standard deviation, RSD)为1.0%。 结论 该方法准确、灵敏度高、重现性好, 适用于保健食品中丙氨酰-酪氨酸含量的测定。

Determination of alanyl tyrosine in health products by high performance liquid chromatography

Objective To establish a method for the determination of alanyl tyrosine in health products by high performance liquid chromatography. Methods Samples were ultrasonically extracted with Primary water solution, separated by Agilent C18 column with acetonitrile-Primary water solution as the mobile phase for gradient elution, which was detected by HPLC and quantified by external standard method. Results Alanyl tyrosine could be separated well, it had a good linear relationship within 0.01?0.05 mg/mL range, the recovery was 95%?105%, RSD was 1.0%. Conclusion This method is accurate, sensitive and reproducible. It is suitable for the determination of alanyl tyrosine in health foods.