| 高效液相色谱法测定磷脂软胶囊中磷脂酰胆碱、磷脂酰肌醇、磷脂酰乙醇胺的含量 |
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| 引用本文： | 王海波,莫紫梅,袁光蔚,廖子祎.高效液相色谱法测定磷脂软胶囊中磷脂酰胆碱、磷脂酰肌醇、磷脂酰乙醇胺的含量[J].食品安全质量检测技术,2020,11(2):579-585. |
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| 作者姓名： | 王海波 莫紫梅 袁光蔚 廖子祎 |
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| 作者单位： | 广西-东盟食品检验检测中心,广西-东盟食品检验检测中心,广西-东盟食品检验检测中心,广西-东盟食品检验检测中心 |
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| 摘 要： | 目的建立高效液相色谱法同时检测磷脂软胶囊中磷脂酰胆碱(phosphatidyl cholines, PC),磷脂酰乙醇胺(phosphatidyl ethanolamine, PE),磷脂酰肌醇(phosphatidylinositol, PI)3种组分的含量。方法样品用甲醇超声提取后,经Bridge?HILIC色谱柱(4.6 mm×150 mm, 5μm)分离,以0.9 mmol/L甲酸铵溶液-乙腈为流动相进行梯度洗脱,流速为1.0 mL/min;柱温30℃;检测波长为205 nm,外标法定量。结果磷脂酰胆碱,磷脂酰乙醇胺,磷脂酰肌醇在浓度0.025～0.250mg/mL范围内线性关系良好,相关系数均大于0.99,检出限分为0.300～1.386 mg/g,定量限为1.002～4.624 mg/g,加标回收率分别为90%～105%,相对标准偏差为1.0%～4.0%。结论该方法高效,便捷,准确度高,适用于磷脂软胶囊中3种磷脂成分的同时检测。
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| 关 键 词： | 磷脂酰胆碱 磷脂酰乙醇胺 磷脂酰肌醇 高效液相色谱 快速检测 |
| 收稿时间： | 2019/11/27 0:00:00 |
| 修稿时间： | 2020/1/6 0:00:00 |
Determination of phosphatidyl cholines, phosphatidyl ethanolamine and phosphatidylinositol in phospholipid soft capsules by high performance liquid chromatography |
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| WANG Hai-Bo,MO Zi-Mei,YUAN Guang-Wei and Liao Zi-Yi.Determination of phosphatidyl cholines, phosphatidyl ethanolamine and phosphatidylinositol in phospholipid soft capsules by high performance liquid chromatography[J].Food Safety and Quality Detection Technology,2020,11(2):579-585. |
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| Authors: | WANG Hai-Bo MO Zi-Mei YUAN Guang-Wei and Liao Zi-Yi |
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| Affiliation: | Guangxi-ASEAN Food Inspection and Testing Center,Guangxi-ASEAN Food Inspection and Testing Center,Guangxi-ASEAN Food Inspection and Testing Center and Guangxi-ASEAN Food Inspection and Testing Center |
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| Abstract: | |
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| Keywords: | phosphatidyl cholines phosphatidyl ethanolamine phosphatidylinositol high performance liquid chromatography rapid detection |
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