| 超临界CO2技术萃取蛋黄磷脂 |
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| 引用本文： | 赖炳森,毛中兴,沈晓京,孙树秦.超临界CO2技术萃取蛋黄磷脂[J].生物化学与生物物理进展,1995,22(4):366-370. |
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| 作者姓名： | 赖炳森 毛中兴 沈晓京 孙树秦 |
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| 作者单位： | 解放军北京医学高等专科学校生物化学教研室;解放军北京医学高等专科学校生物化学教研室;解放军北京医学高等专科学校生物化学教研室;解放军北京医学高等专科学校生物化学教研室 |
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| 基金项目： | 全军“八五”医药卫生科研基金资助项目. |
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| 摘 要： | 采用新型物理分离技术──超临界CO2萃取法,提取天然蛋黄粉中的磷脂.在40MPa,先去除蛋黄粉中甘油三酯和胆固醇,再萃取磷脂.结果显示,磷脂纯度为95%,N/P比值为1.003,λmax=214nm,薄层层析显示磷脂着色点清晰,并去除了绝大部分甘油三酯和胆固醇.此法操作简单、产品质量高、安全和不污染环境,还可得到天然纯蛋黄油和蛋白.
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| 关 键 词： | 超临界CO2萃取 磷脂 蛋黄 |
| 收稿时间： | 1994/9/19 0:00:00 |
| 修稿时间： | 1994/12/26 0:00:00 |
The Extraction of Yolk Phospholipid by the Technique of Supercritical Fluid CO2 Extraction |
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| Lai Bingsen,Mao Zhongxing,Shen Xiaojing and Sun Shuqin.The Extraction of Yolk Phospholipid by the Technique of Supercritical Fluid CO2 Extraction[J].Progress In Biochemistry and Biophysics,1995,22(4):366-370. |
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| Authors: | Lai Bingsen Mao Zhongxing Shen Xiaojing and Sun Shuqin |
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| Affiliation: | Department of Biochemistry, Beijing Medical College of PLA;Department of Biochemistry, Beijing Medical College of PLA;Department of Biochemistry, Beijing Medical College of PLA;Department of Biochemistry, Beijing Medical College of PLA |
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| Abstract: | Phospholipid(PL)has higher medical and nutritional values and widely applied in medical,food,Chemical industry field etc. The natural PL of yolk powder was extracted by supercritical fluid CO2 extraction(SFE-CO2)technique. The phosphorus,nitrogen and PL contents,the N/P molar ratio,the triglyceride and cholesterol of the extraction were measured by means of biochemical method,thin layer chromatography,and spectral analysis. The results showed that extracted yolk PL is 95%in purity and its N/P molar ratio is 1.003. So the SFE-CO2 technique can be used to obtain yolk PL with higher purity quality. |
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| Keywords: | supercritical fluid CO_2 extraction(SFE-CO_2) phospholipid(PL) yolk |
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