CCK-8对LPS攻击小鼠IL-1β、IL-6、IL-4、IL-10表达的影响

目的： 观察LPS攻击小鼠IL-1β、IL-6、IL-4、IL-10的动态变化规律及八肽胆囊收缩素（CCK-8）对其表达的影响。方法: 将小鼠分为4组：对照组、LPS组（腹腔注射LPS）、LPS+CCK-8组（注射LPS前 30 min 腹腔注射CCK-8）及CCK-8组（单独注射CCK-8）。用ELISA及PT-PCR方法检测各组小鼠血清、肺组织中IL-1β、IL-6、IL-4、IL-10的含量及mRNA的表达情况。结果: LPS攻击可使小鼠血清及肺组织中 IL-1β、IL-6、IL-4、IL-10蛋白及mRNA的表达增加，预先注入CCK-8可显著抑制IL-1β、IL-6的表达，并使 IL-4、IL-10的表达进一步增加。结论: CCK-8可能通过抑制LPS攻击小鼠IL-1β、IL-6的表达和进一步增加 IL-4、IL-10的表达参与抗炎反应过程，从而减轻LPS引起的肺组织炎症反应。

Effects of cholecystokinin octapeptide on expression of IL-1β, IL-6, IL-4 and IL-10 in lipopolysaccharide-attacked mice

AIM:To observe the effects of cholecystokinin octapeptide (CCK-8) on the expression of proinflammatory cytokines IL-1β, IL-6 and anti-inflammatory cytokines IL-10, IL-4 in LPS- attacked mice. METHODS:Kunming mice were randomly assigned and injected intraperitoneally with LPS alone or/and CCK-8 at different time points. The expression of IL-1β, IL-6, IL-10 and IL-4 in the serum and lung tissues were assayed by ELISA and RT-PCR. RESULTS:The expression of IL-1β, IL-6, IL-10 and IL-4 were upregulated in LPS-attacked mice. Pre-treatment of CCK-8 decreased both IL-1β and IL-6 expression and augmented IL-10 and IL-4 expression in LPS-attacked mice. CONCLUSIONS:CCK-8 exerts an anti-inflammatory effect by inhibiting the expression of IL-1β, IL-6 and increasing the expression of IL-10, IL-4 in LPS-attacked mice, which could alleviate the inflammatory response in lung tissue.