| GST-hBLyS融合蛋白基因的构建及其在大肠杆菌中的表达 |
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| 引用本文： | 张志方,张春艳,付涌水,林学颜,潘敬运.GST-hBLyS融合蛋白基因的构建及其在大肠杆菌中的表达[J].中山大学学报(自然科学版),2002,41(3):72-76. |
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| 作者姓名： | 张志方 张春艳 付涌水 林学颜 潘敬运 |
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| 作者单位： | 1. 广州血液中心,广东,广州,510095
2. 中山大学中山医学院,广东,广州,510089 |
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| 摘 要： | 根据hBLyS (humanBlymphocytestimulator)基因序列设计合成特异性引物 ,用RT_PCR从人外周血淋巴细胞扩增出 85 8bp的hBLyS基因 ,并将其插入到融合蛋白原核表达载体 pGEX_4T_1中 ,得到重组表达质粒pGEX_4T_1/hBLyS。把此重组质粒转化大肠杆菌BL2 1,经用IPTG诱导 ,表达出了GST_hBLyS融合蛋白。
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| 关 键 词： | 人BLyS 基因表达 融合蛋白 |
| 文章编号： | 0529-6579(2002)03-0072-04 |
| 修稿时间： | 2001年10月26 |
Construction of Recombinant Plasmid of GST-hBLyS Fusion Protein Gene and Expression of GST-hBLyS Fusion Protein in E.coli BL21 |
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| ZHANG Zhi_fang ,ZHANG Chun_yan ,FU Yong_shui ,LIN Xue_yan ,PAN Jing_yun.Construction of Recombinant Plasmid of GST-hBLyS Fusion Protein Gene and Expression of GST-hBLyS Fusion Protein in E.coli BL21[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2002,41(3):72-76. |
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| Authors: | ZHANG Zhi_fang ZHANG Chun_yan FU Yong_shui LIN Xue_yan PAN Jing_yun |
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| Affiliation: | ZHANG Zhi_fang 1,ZHANG Chun_yan 1,FU Yong_shui 2,LIN Xue_yan 1,PAN Jing_yun 1 |
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| Abstract: | The human B lymphocyte stimulator (hBLyS) gene, a 858 bp DNA fragment, was amplified by RT_PCR using a pair of primers which were designed and synthesized according to the hBLyS gene sequence in GenBank. The hBLyS gene was cloned into pGEX_4T_1 plasmid,a GST expression Vector. The recombinant expression plasmid of pGEX_4T_1/hBLyS was transformed into E coli BL21. The recombinant GST_hBLyS fusion protein was expressed after induced by IPTG in E coli BL21. |
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| Keywords: | hBLyS gene expression fusion protein |
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