| 荷叶生物碱与DNA相互作用的光谱法研究 |
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| 引用本文： | 单斌,王素燕,张卫国.荷叶生物碱与DNA相互作用的光谱法研究[J].韶关学院学报,2008,29(12). |
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| 作者姓名： | 单斌 王素燕 张卫国 |
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| 作者单位： | 韶关学院食品科学系,广东韶关,512005 |
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| 基金项目： | 广东省科技攻关项目(2008B020200001) |
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| 摘 要： | 采用荧光光谱、紫外-可见光谱和盐效应等手段.研究了荷叶生物碱提取物与小牛胸腺DNA之间的相互作用,并在pH 7.4的Tris-HCl介质中.以中性红为荧光探针对作用机理进行了研究.结果表明:在生理条件下(pH= 7.4),荷叶生物碱提取物与DNA发生作用方式为混合方式,嵌插与沟槽作用是两种主要作用方式,DNA对荷叶生物碱提取物荧光猝灭属于静态猝灭。测得其结合常数为3.765×106 L/mol.
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| 关 键 词： | 荷叶 生物碱 小牛胸腺DNA 荧光光谱 |
Studies on the interaction between alkaloids extractive and DNA by spectrometry |
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| SHAN Bin,WANG Su-yan,ZHANG Wei-guo.Studies on the interaction between alkaloids extractive and DNA by spectrometry[J].Journal of Shaoguan University(Social Science Edition),2008,29(12). |
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| Authors: | SHAN Bin WANG Su-yan ZHANG Wei-guo |
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| Affiliation: | (Department of Food Science; Shaoguan University; Shaoguan 512005; Guangdong; China); |
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| Abstract: | The interaction between alkaloids extractive and DNA was determined by fluoresence spectrum,vio- let-absorption spectrometric spectrum and salt effect experimental.The mechanism of the interation was also in- vestigated by fluorescence spectroscopy using neutral red as a spectral probe in Tris-HCl buffer solution(pH= 7.4).The results showed that alkaloids extractive can react with DNA under physiological condition(pH=7.4). The interaction mode was of a mixed one that the inlay and groove binding were the tw... |
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| Keywords: | lotus leaves alkaloids ct-DNA fluorescence spectra |
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