线性离子阱-傅立叶变换离子回旋共振质谱的结构原理及在蛋白质组学研究中的应用

介绍了线性离子阱-傅立叶变换离子回旋共振质谱(LTQ-FT-MS)的结构和特点,并以美国热电集团的LTQ-FT-MS为例,介绍了该仪器在蛋白质组学研究中的应用。LTQ-FT-MS将液相色谱-质谱技术联用,将测量精度提高到1~2μg/g,可以显著提高全新肽链测序的确定性。FT-MS可通过电子捕获解离技术对天然蛋白质的结构进行鉴定;还可用于蛋白质分子的“由上而下”的多级质谱分析,对高度同源的蛋白质序列进行鉴定。

Structural Principle of the Linear Ion Trap-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Its Application in Proteomics

The structural principle of the linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry(LTQ-FT-MS)was introduced using the LTQ-FT-MS produced by Thermo Corporation as an example.Also its application in proteomics was introduced briefly.Using liquid chromatographymass spectrometry(LC-MS) coupling technique,LTQ-FT-MS could provide high measure accuracy,routinely at 1-2 μg/g,which could significantly enhance the validation of peptide identification.This FT-MS could use electron capture dissociation technique,getting more information in structural identification.The top-down multiple mass spectrometer analysis of protein molecules could also be applied,which could identify some protein sequence from high homogeneity family.