| 多环芳烃类物质对细胞色素P450 1A1的诱导表达 |
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| 引用本文： | 谢丹君,徐晶莹,诸葛淳淳,吕立夏,张敬法,徐国彤.多环芳烃类物质对细胞色素P450 1A1的诱导表达[J].同济大学学报(医学版),2013,34(2):1-5. |
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| 作者姓名： | 谢丹君 徐晶莹 诸葛淳淳 吕立夏 张敬法 徐国彤 |
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| 作者单位： | [1]同济大学医学院再生医学系临床视觉科学实验室,上海200092 [2]同济大学医学院眼科研究所,上海200072 [3]中国科学院上海生命科学研究院健康科学研究所,上海200025 |
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| 基金项目： | 国家自然科学基金（31201084）;国家自然科学基金（31071199）;上海市自然科学基金（12ZR1434400） |
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| 摘 要： | 目的探讨多环芳香烃类化合物(polycyclic aromatic hydrocarbons,PAHs)对细胞色素P450 1A1(CYP 1A1)的诱导表达情况。方法以人肺癌细胞系A549为研究对象,选取16种含量较多的典型的PAHs暴露给药。MTT方法检测不同药物浓度下细胞的存活率以选择合适的给药浓度;在该浓度药物处理24 h后,收集各组细胞的总RNA及蛋白,用实时荧光定量(Real-Time)PCR及Western印迹法检测CYP1A mRNA和蛋白的表达。结果 MTT结果显示,PAHs浓度在0.2 mol/L时,细胞存活率为50%～60%,选用此浓度作为后续实验的给药浓度。与正常对照组相比,fluorene、2,3-benzanthracene、perylene、acenaphthylene和benzoa]pyrene能显著诱导CYP1A1 mRNA和蛋白的表达(P<0.05)。结论 fluorene、2,3-benzanthracene、perylene、acenaphthylene和benzoa]pyrene等烟草烟气内存在的化合物对细胞色素酶CYP1A1具有诱导表达作用。
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| 关 键 词： | 细胞色素P450 1A1 多环芳香烃类化合物 肺肿瘤 |
Induction of cytochrome P450 1A1 expression in A549 cells by polycyclic aromatic hydrocarbons |
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| XIE Dan-jun,XU Jing-ying,ZHUGE Chun-chun,LU Li-xi,ZHANG Jing-fa and XU Guo-tong.Induction of cytochrome P450 1A1 expression in A549 cells by polycyclic aromatic hydrocarbons[J].Journal of Tongji University(Medical Science),2013,34(2):1-5. |
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| Authors: | XIE Dan-jun XU Jing-ying ZHUGE Chun-chun LU Li-xi ZHANG Jing-fa and XU Guo-tong |
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| Affiliation: | 1. Laboratory of Clinical Visual Sciences, Dep. of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China; 2. Tongji Eye Institute, Tongji University School of Medicine, Shanghai 200072, China; 3. Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China) |
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| Abstract: | Objective To investigate the inductive effect of polycyclic aromatic hydrocarbons （PAHs） on the expression of cytochrome P450 1A1 in A549 cells. Methods PAHs toxicity was screened by MTT assay in pulmonary epithelial A549 cells, and the mRNA and protein expression of cytochrome P450 isozymes （CYP） 1A was analyzed by Real-Time-PCR and Western blot,respectively. Results The PAHs concentration at 0.2 mol/L was determined by cell viability assay for induction screening. Five PAHs, including fluorene, perylene, acenaphthylene, 2,3-benzanthracene, benzo a] pyrene were identified as CYP1 A1 inducers both in mRNA and protein expression level （P 〈 0.05）. Conclusion In this study five PAHs have been identified as CYP1A1 inducers. |
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| Keywords: | cytochrome P450 1 A1 polycyclic aromatic compounds lung cancer |
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