氧化苦参碱对鼻咽癌耐药细胞株HNE-1(200)耐药逆转机制的研究

目的:研究天然有效成分氧化苦参碱对鼻咽癌耐药细胞株HNE-1(200)耐药逆转的机制。方法:以放射线照射诱导的鼻咽癌耐药细胞株HNE-1(200)为研究对象,采用免疫细胞化学法、免疫印迹(Western Blot)法检测细胞P-糖蛋白(P-gp)表达的变化,用逆转录-聚合酶链反应(RT-PCR)检测多药耐药1基因(mdr1)的mRNA的改变。结果:氧化苦参碱作用于HNE-1(200)细胞24h后,免疫细胞化学方法和Western Blot显示:人鼻咽癌(P-gp)表达下调;RT-PCR显示:HNE-1、HNE-1(200)细胞的mdr1表达无差异。结论:氧化苦参碱能通过下调P-gp表达,在一定程度上逆转耐药,但是mRNA表达并无变化,说明鼻咽癌多药耐药引起P-gp表达上调受多种机制控制。

Reversal of Multidrug Resistance in NPC HNE-1(200)Cell Line by Oxymatrine

OBJECTIVE： To study the reversal mechanism of oxymatrine on multidrug resistance in the NPC HNE- 1 （200） cell line. METHODS： The multidrug resistant HNE - 1（200） subline was derived from an HNE- 1 cell line after exposure to fractionated X - irradiation. The expression of P - gp was tested by immunocytochemical method and western blot and that of mdrl mRNA was measured by RT- PCR. RESULTS： After 24- hour treatment with oxymatrine, both the immunocytochemical method and western blot showed a decrease in the expression of P- gp. However, RT- PCR measurement showed that the expression of mdrl mRNA of HNE- 1 and HNE- 1（200） was nonsignficant. CONCLUSION： Oxymatrine can reverse the multidrug resistance of NPC HNE- 1（200） cell line to some degree by down- regulating the expression of P- gp. However, this medicine did no effect on the expression of mdrl mRNA, suggesting the up-regulation of the expression of P- gp is under the control of multiple mechanism.