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| 薄层扫描法测定注射用硫酸庆大霉素中3组分的含量 | |
| 引用本文： | 贾忠,袁继勇,吕东煜.薄层扫描法测定注射用硫酸庆大霉素中3组分的含量[J].中国药房,2005,16(3):217-218. |
| 作者姓名： | 贾忠 袁继勇 吕东煜 |
| 作者单位： | 1. 兰州市肺科医院药剂科,兰州市,730046 2. 甘肃省人民医院药剂科,兰州市,730000 3. 兰州医学院医学工程系,兰州市,730000 |
| 摘 要： | 目的 :建立注射用硫酸庆大霉素中3组分含量的快速测定方法。方法 :采用薄层扫描法 ,以甲醇 -氯仿 -28 %氨水 (1∶1∶1)为展开剂 ,新配制的茚三酮的乙醇 -冰醋酸溶液为显色剂 ,测定波长为500nm。结果 :注射用硫酸庆大霉素中3组分的点样量均在2μg～12μg 范围内线性关系良好 (r1=0. 9993、r2=0. 9996、r1a=0 .9991)。结论 :薄层扫描法可作为注射用硫酸庆大霉素中3组分含量的快速测定方法。 |
| 关 键 词： | 注射用硫酸庆大霉素 薄层扫描法 含量测定 |
| 文章编号： | 1001-0408(2005)03-0217-02 |
| 修稿时间： | 2004年10月25 |
Content Determination of Three Components in Gentamicin with Scan- TLC |
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| JIA Zhong.Content Determination of Three Components in Gentamicin with Scan- TLC[J].China Pharmacy,2005,16(3):217-218. | |
| Authors: | JIA Zhong |
| Abstract: | OBJECTIVE:To establish a rapid determination method for gentaminin active components.METHODS:Scan-TLC procedure was applied.A mixture of methanol-chloroform-28%ammonium hydroxide(1∶1∶1)was taken as developing reagent and ninhydrin in a mixtrue solvent as reveal reagent.The detecting wavelength was500nm.RESULTS:The amounts of three components in gentamincin per spot were linear in the range of2μg～12μg(r 1 =0.9993、r 2 =0.9996、r 1a =0.9991).CONCLUSION:This scan-TLC technique is recommendable for determination of gentamincin components. |
| Keywords: | Gentamicin Injection Scan-TLC Determination of contents |
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