红参对乳酸菌体外增菌的影响

采用比浊法和活菌计数法研究在基础培养基/菊芋汁基础培养基中添加不同量低聚木糖、低聚果糖、红参提取液及其酸水解物对鼠李糖乳杆菌AS 1.2466T/保加利亚乳杆菌ATCC 11842生长情况的影响。结果表明:不同添加物对鼠李糖乳杆菌AS 1.2466T的增菌效果为红参提取液>红参酸水解物>低聚木糖>低聚果糖。当红参提取液添加量为12.50%时,鼠李糖乳杆菌AS 1.2466T的最大OD600值为1.172,活菌数为2.72×10^8 CFU/mL,比基础培养基中最大活菌数7.75×10^7 CFU/mL增加1个数量级。对保加利亚乳杆菌ATCC 11842的增菌效果为红参提取液>低聚木糖>红参酸水解物>低聚果糖。当红参提取液添加量为6.25%时,保加利亚乳杆菌ATCC 11842的最大OD600值为0.627,活菌数为4.43×10^8 CFU/mL,比菊芋汁基础培养基中最大活菌数1.48×10^7 CFU/mL增加1个数量级。红参提取液比低聚木糖、低聚果糖具有较好的增菌效果,将红参作为益生元研究其益生功能,为研究红参对肠道微生物的影响提供试验依据。

Effects of Red Ginseng on the Proliferation of Lactic Acid Bacteria in Vitro

In this study,the turbidimetry and viable count methods were used to study the growth of Lactobacillus rhamnosus AS 1.2466T/Lactobacillus bulgaricus ATCC 11842 in basic medium/Jerusalem artichoke juice base medium with different amounts of xylo-oligosaccharides,oligosaccharides,red ginseng liquid and its acid hydrolyzate.The results showed that the enrichment effect of different additives on Lactobacillus rhamnosus AS 1.2466T is red ginseng liquid medicine>red ginseng acid hydrolyzate>xylo-oligosaccharide>oligofructose.The OD600 value of Lactobacillus rhamnosus AS 1.2466T was 1.172 when the amount of red ginseng was 12.50%;and the number of viable cells was 2.72×10^8 CFU/mL,which was an order of magnitude higher than that of the basic medium 7.75×10^7 CFU/mL.The enrichment effect of prebiotics on Lactobacillus bulgaricus ATCC 11842 is red ginseng liquid medicine>xylo-oligosaccharides>red ginseng acid hydrolyzate>oligofructose.The OD600 value of Lactobacillus bulgaricus ATCC 11842 was 0.627 when the amount of red chemical solution was 6.25%;and the number of viable cells was 4.43×10^8 CFU/mL,which was an order of magnitude higher than the maximum viable count of 1.48×10^7 CFU/mL in the basal medium of Jerusalem artichoke.In summary,we found that red ginseng has a better enrichment effect than xylo-oligosaccharides or oligofructose.It can be used as a prebiotics to further study its prebiotics function,which can provide a basis on the study of the effect of red ginseng on intestinal microbes.