| 高大毛霉制取果胶酶发酵条件实验 |
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| 引用本文： | 顾红燕,齐鸿雁,张洪勋.高大毛霉制取果胶酶发酵条件实验[J].过程工程学报,2002,2(3):252-256. |
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| 作者姓名： | 顾红燕 齐鸿雁 张洪勋 |
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| 作者单位： | 中国科学院生态环境研究中心,北京,100085 |
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| 基金项目： | 中国科学院生态环境研究中心知识创新课题资助项目(编号:RCEES-9904) |
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| 摘 要： | 对高大毛霉(Mucor mucedo)制取果胶酶的发酵条件和酶的基本性质进行了研究. 发酵培养基组成为(g/L)：小麦麸皮50，葵盘粉30，(NH4)2SO4 30，KH2PO4 2.5，MgSO4×7H2O 0.5，NaNO3 0.2，FeSO4×7H2O 0.01. 在培养温度30℃、初始pH 5.7、转速240 r/min条件下摇瓶培养3 d，酶活力达到275 U/ml. 该酶最适pH为5.0，最适作用温度为40℃，在pH为3.0~7.0范围内稳定.
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| 关 键 词： | 高大毛霉 果胶酶 发酵条件 |
| 文章编号： | 1009-606X(2002)03-0252-05 |
| 修稿时间： | 2001年12月3日 |
Fermentation Conditions of Pectinase by Mucor mucedo |
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| GU Hong-yan,QI Hong-yan,ZHANG Hong-xun.Fermentation Conditions of Pectinase by Mucor mucedo[J].Chinese Journal of Process Engineering,2002,2(3):252-256. |
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| Authors: | GU Hong-yan QI Hong-yan ZHANG Hong-xun |
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| Abstract: | The fermentation conditions of pectinase by Mucor mucedo and its enzymic properties were studied. The composition of fermentation medium was (g/L): wheat bran 50, sunflower calathide powders 30, (NH4)2SO4 30, KH2PO4 2.5, MgSO47H2O 0.5, NaNO3 0.2, FeSO47H2O 0.01. The pectinase activity can reach 275 U/ml after 3 d shaker culture under the conditions of 240 r/min, 30oC and initial pH 5.7. The optimum temperature and pH for the pectinase activity were 40oC and 5.0 respectively. The pectinase was stable in the pH range from 3.0 to 7.0. |
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| Keywords: | Mucor mucedo pectinase fermentation conditions |
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