| 抗人结肠癌单克隆抗体MC_6的制备及其相应抗原的免疫组化定位研究 |
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| 引用本文： | 张京航,贺武,宋今丹.抗人结肠癌单克隆抗体MC_6的制备及其相应抗原的免疫组化定位研究[J].标记免疫分析与临床,1997(4). |
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| 作者姓名： | 张京航 贺武 宋今丹 |
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| 作者单位： | 中国医科大学微生物学教研室!沈阳，110001，中国医科大学细胞生物学卫生部重点实验室!沈阳，110001，中国医科大学细胞生物学卫生部重点实验室!沈阳，110001 |
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| 摘 要： | 本研究利用手术中取得的新鲜结肠癌组织免疫Balb/c小鼠。制得5株能持续、稳定分泌抗体的杂交瘤细胞系。其中HC6株杂交瘤细胞经过180天连续培养，单克隆抗体MC6分泌稳定，选择性强，效价高。免疫组化检测发现，MC6相应抗原在结肠癌组织及结肠癌细胞系高度表达（90％～100％），显著高于其它癌组织，癌旁和正常粘膜及癌细胞系（0％～44．4％）；中度以上染色者占阳性者总数80％以上，主要分布于细胞膜上，且除癌细胞膜和胞浆呈阳性反应外，腺腔内粘液亦可见不同程度着色。表明MC6相应抗原是一种在结肠癌细胞膜上占优势的抗原，并可脱落至腺腔。此单克隆抗体的成功研制为发现新的结肠癌生化标志，为结肠癌早期诊断及术后病情监测提供了可能性。
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| 关 键 词： | 免疫组化细胞融合 结肠肿瘤 抗体单克隆 |
A New Monoclonal Antibody to Human Colonic Carcinoma and Immunohistochemical Localization of Its Related Antigen |
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| Zhang Jinghang, He Wu, Song Jindan.A New Monoclonal Antibody to Human Colonic Carcinoma and Immunohistochemical Localization of Its Related Antigen[J].Labeled Immunoassays and Clinical Medicine,1997(4). |
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| Authors: | Zhang Jinghang He Wu Song Jindan |
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| Abstract: | Five hybridomas capable of persistently secreting monoclonal antibodies were obtained with fresh carcinoma tissue of clolon as antigen. Among them, hybridoma MC6 was serially cultured for 180 days and produced a monoclonal antibody MC6 with high stability, specificity and titer. Indirect immunofluorecent and immunohistochemical staining demonstrated that MC6 related antigen was expressed in more than 90 % of cancer tissues or cell lines of colonic carcinoma origin, being significantly higher than that from other kinds of tumors. Double immunodiffusion showed no cross - reactivity between MC6 related antigen and CEA. |
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