Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var. grubii,Cryptococcus neoformans var. neoformans,Cryptococcus gattii,and Hybrids |
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| Authors: | Xiaobo Feng Xiaohua Fu Bo Ling Lei Wang Wanqing Liao Zhirong Yao |
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| Affiliation: | Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicinea;School of Environmental Science and Engineering, Tongji Universityb;Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Institute of Medical Mycology, Changzheng Hospital Affiliated to Second Military Medical University,c Shanghai, China |
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| Abstract: | A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies. |
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