Improved analysis of sinigrin in Ethiopian mustard |
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| Authors: | Deyun Yuan Youn Young Shim Martin JT Reaney Venkatesh Meda |
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| Affiliation: | 1. Department of Chemical and Biological Engineering, University of Saskatchewan, Saskatoon, SK, Canada;2. Department of Plant Science, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5A8, Canada;3. Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University, Guangzhou, Guangdong, 510632, China |
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| Abstract: | An ion‐pair reversed‐phase high‐performance liquid chromatography (HPLC) method was developed for the analysis of sinigrin in Ethiopian mustard (Brassica carinata A. Braun) seed and seed fractions. Separation was compared on several RP‐HPLC columns (Inertsil® ODS‐4 C18 and ZORBAX® Eclipse XDB‐C18) with an isocratic eluent containing 100% aqueous (aq.) tetramethylammonium bromide (10 mm , pH 5.0). Sinigrin retention was affected by HPLC variables including the type of ion‐pair reagent, buffer strength and pH, acetonitrile concentration, column temperature and eluent flow rate. Partial validation demonstrated this optimised chromatographic condition to be linear, accurate and precise. Multistage extraction using 70% (v/v) aq. methanol was more efficient than 50% (v/v) aq. acetonitrile. In addition, the matrix effect and recovery rate as well as processing efficiency of the analytical protocol were determined. This method is suitable for high throughput analysis of sinigrin in Ethiopian mustard seed and seed fractions. |
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| Keywords: |
Brassica carinata
Ethiopian mustard high‐performance liquid chromatography matrix effect retention factor (k) sinigrin |
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