西瓜皮培养液对草履虫种群生长的影响

以西瓜皮为原料，探讨其培养液对草履虫种群生长的影响。将新鲜西瓜皮配成2%、4%、6%、8%、10%、12%、14%、16%、18%、20%的培养液，进行草履虫培养试验，在接种后第4、7、10、16、19、30天采用微量计数法对草履虫进行计数，培养期为30 d。试验结果表明：2%、4%、6%、8%的西瓜皮培养液中草履虫达到最大密度值一般在接种后的10 d，其最大种群密度分别为（533.3626.47）、（666.7015.46）、（920.1016.47）、（880.5746.35）mL-1；而10%、12%、14%、16%的西瓜皮培养液达到最大密度一般在接种后7 d，其最大种群密度分别为（640.5324.21）、（741.3228.32）、（862.0735.19）、（912.8635.86）mL-1。16 d时，所有培养液中草履虫密度开始急速下降；到19 d时，10%、12%、14%、16%的培养液中已经检测不到草履虫了；到30 d时，只有在2%、4%、6%、8%中检测到少量草履虫。从整个培养期来看，6%和8%的西瓜皮培养液培养草履虫效果最佳，最佳培养时间为7~10 d。本试验旨在为生物试验教学及科学研究中培养草履虫提供快速、经济、可行的培养方法。

The Effects of Watermelon Rind on Population Growth of Paramoecium Caudatum

Ten kinds of mediums were used in the study,they were respectively 2%,4%,6%,8%,10%,12%,14%,16%,18%,20% media of watermelon rind, being used to culture paramecium (Paramecium caudatum),to compare the effects of different mediums of watermelon rind on the population growth of paramecium and select the suitable mediums for culturing paramecium.After culturing paramecium of 4 d,7 d,10 d,16 d,19 d,30 d, micro-counting methodwere used and cultivation period was 30 days. The results showed that 2%,4%,6%,8% of watermelon rind mediums, paramecium maximum density were respectively (533.3626.47), (666.7015.46), (920.1016.47) and (880.5746.35) mL-1 at 10 days. The maximum density of concentration of 10%,12%,14%,16% watermelon rind mediums were respectively (640.5324.21), (741.3228.32),(862.0735.19) and (912.8635.86)mL-1 at 7 days. At 16 days, paramecium density in all cultures began to fell sharply at 10%,12%,14%,12% of medium. At 30 day, 2%,4%,6%,8% of watermelon rind mediums could detect a few paramecia. From whole period, the most suitable concentration of watermelon rind mediums were 6% and 8% and the best time was 7 to 10 days. This experiment was intended to cultivate paramecium in biological experiment teaching and scientific research to provide fast, economic and feasible methods for culturing paramecium.