血细胞分析仪对外周血有核红细胞计数性能的评价

目的评价Sysmex XE-2100血细胞分析仪计数外周血有核红细胞（NRBC）的性能。方法选择门诊与住院患者乙二胺四乙酸二钾抗凝血标本,采用Sysmex XE-2100血细胞分析仪与手工显微镜方法计数NRBC,并对结果进行比较。结果血细胞分析仪与手工显微镜方法之间相关性良好,相关系数为0.938 4;58份标本仪器法NRBC在0.00%～0.99%标本中14份（24.1%）手工法NRBC结果为0.00%。24份仪器法NRBC结果为0.00%的标本中22份（91.2%）手工法小于或等于1.00%;仪器法重复性明显好于手工法,NRBC平均CV为8.0%。结论 Sysmex XE-2100血细胞分析仪与手工显微镜法之间相关性较好;仪器法重复性明显好于手工法;尽管出现少量仪器法与镜检法不一致结果,但均伴有其他白细胞和（或）血小板异常报警提示,也进行了显微镜复核。

Evaluation of the performance of Sysmex XE-2100 for detection of nucleated red cells in peripheral blood

Objective To evaluate the performance of Sysmex XE-2100analyzer for detection of nucleated red cells（NRBC）in peripheral blood.Methods Peripheral blood samples,collected from in-patients and out-patients and anticoagulated by ethylene diamine tetraacetate dipotassium,were detected by Sysmex XE-2100analyzer and manual method using microscope and Wright-Giemsa stained smear.Then the results were analyzed.Results Correlation coefficient of the results detected by analyzer and manual method was 0.938 4.Among the 58samples,with the NRBC percentage of 0.00%-0.99%detected by analyzer,there were 14samples with NRBC percentage of 0.00%,detected by manual method,accounting for 24.1%.Among the 24samples,with the NRBC percentage of 0.00%detected by analyzer,there were 22sampels with NRBC percentage less than or equal with 1.00%,accounting for 91.2%.The reproducibility of analyzer was better than manual method,and the coefficient of variability of the former was 8.0%.Conclusion There could be fine correlation between the Syxmex XE-2100analyzer and manual method.The reproducibility of analyzer might be better than manual method.A few samples could be with different results,detected by analyzer and manual method,but the analyzer could supply warning flag to perform manual review.