口腔鳞癌组织中Survivin蛋白的表达及与血管生成的关系

背景与目的:Survivin蛋白主要通过抑制Caspase-3、Caspase-7阻断细胞凋亡过程,在多种常见恶性肿瘤组织中有表达。本研究拟探讨Survivin在口腔鳞癌发生发展中的作用及与血管生成的关系。方法:采用免疫组织化学SP法,检测8例正常口腔粘膜、14例上皮异常增生性白斑、47例鳞癌组织中Survivin的表达及鳞癌中CD34的表达,并计数微血管密度(MVD),分析口腔鳞癌中Survivin与MVD及临床病理特征的关系。结果:Survivin在正常口腔粘膜、异常增生性白斑及鳞癌组织中的阳性率分别为0%(0/8)、14.29%(2/14)和55.32%(26/47);经免疫组化半定量分析,鳞癌组Survivin表达强于正常口腔粘膜组及异常增生性白斑组(P<0.05),而后两组间无显著差别(P>0.05)。Survivin在中-低分化鳞癌组中的表达强于高分化鳞癌组(P<0.05);在有淋巴结转移组中的表达(71.43%)高于无淋巴结转移组(38.89%)(P<0.05)。口腔鳞癌中,随Survivin表达增强(-, ,2 ,3 ),MVD逐渐增高(分别为25.87±12.10,28.70±7.69,35.42±10.09,41.13±9.62)(P<0.05)。结论:Survivin蛋白在口腔鳞癌组织中表达上调;Survivin与口腔鳞癌的分化程度、淋巴结转移有关,并与MVD关系密切,提示Survivin与口腔鳞癌的发生发展过程有关。

Expression of survivin and its correlation to angiogenesis in oral squamous cell carcinoma

BACKGROUND & OBJECTIVE: Survivin can block the cell apoptosis through inhibiting the functions of Caspase-3 and Caspase-7, and selectively overexpresses in common human cancers. This study was designed to investigate the effects of Survivin on tumorigenesis and development of oral cancer and its correlation to angiogenesis. METHODS: The expression of Survivin in 8 specimens of normal oral mucosa, 14 specimens of dysplastic leukoplakia and 47 specimens of oral squamous cell carcinoma (OSCC), and the expression of CD34 in the 47 specimens of OSCC was detected by SP immunohistochemistry. Microvessel density (MVD) was also assessed. The correlations of Survivin expression to MVD and clinicopathologic features of the OSCC patients were analyzed. RESULT: The positive rates of Survivin were 0% in normal oral mucosa, 14.24% in dysplastic leukoplakia, and 55.32% in OSCC. Survivin expression was significantly stronger in OSCC than in normal oral mucosa and dysplastic leukoplakia (P < 0.05), while there was no difference between the last 2 groups (P > 0.05). Survivin expression was significantly stronger in moderately and poorly differentiated OSCC than in well differentiated OSCC (P < 0.05); the positive rate of Survivin was significantly higher in OSCC with lymph node metastasis than in OSCC without lymph node metastasis(71.43% vs. 38.89%, P < 0.05). In OSCC, MVD was increased (25.87 +/- 12.10, 28.70 +/- 7.69, 35.42 +/- 10.09, 41.13 +/- 9.62, respectively) along with the strengthened Survivin expression (-, +, 2+, 3+) (P < 0.05). CONCLUSION: Survivin expression is up-regulated in OSCC, and closely related with tumor cell differentiation, lymph node metastasis, and MVD; it may play an important role in tumorigenesis and development of OSCC.