具有生物活性的川牛膝果聚糖的结构

从传统中药川牛膝(Cyathula offcinalis kuan)中分离提取到了一种具有生物活性的多糖RCP.核磁共振、甲基化分析、还原裂解和GC-MS分析揭示了RCP是一高度分支的果聚糖,它以(2→1)连接为骨架,其上有大量的(2→6)连接的分支,且属于新蔗果三糖系列.在93.17%果糖残基中,24.15%是末端果糖,26.24%是1-连接果糖,20.46%是6-连接果糖.在6.83%的葡萄糖残基中,2.14%是末端葡萄糖,4.69%是6-连接葡萄糖.RCP的平均聚合度是15.

Structure of a Bioactive Fructan from the Root of Cyathula officinalis

The structure of a bioactive fructan RCP isolated from the traditional Chinese medicinal herb Cyathula officinalis Kuan was determined by NMR, methylation, reductive-cleavage and GC-MS analyses. It was a highly branched fructan possessing a (2→1)-linked b-D-fructofuranosyl (Fruf ) backbone with (2→6)-linked b-D-Fruf side chains, belonging to neokestose family. Of the 93.17% b-D-Fruf residues, 24.15% was terminal, 26.24% was 1-linked, 20.46% was 6-linked, and 22.32% was 1,6-linked. Of the 6.83% a-D-glucopyranosyl (Glcp) residues, 2.14% was terminal and 4.69% was 6-linked. A structural model was postulated for a degree of polymerization (DP) of 15.