实验用2型糖尿病大鼠模型及其肾病观察

目的研究高脂高糖喂养加小剂量链脲佐菌素诱导的2型糖尿病大鼠模型的可行性及其肾病特点。方法SD大鼠高脂高糖喂养8周后,腹腔注射STZ(35mg/kg)诱导糖尿病大鼠,观察血糖,空腹胰岛素,血脂,肾功能,并行光镜及电镜观察肾脏结构改变。结果高脂高糖喂养加小剂量STZ诱导的糖尿病大鼠的体重,血糖,血脂较对照组明显增高,胰岛素敏感指数下降。糖尿病12周时,肾肥大指数、尿微量白蛋白、尿素氮、肌酐、肾小球截面计、基质面积、基质面积比均较正常对照组升高,而毛细血管面积比下降。结论高脂高糖喂养加小剂量STZ诱导的糖尿病大鼠符合2型糖尿病发病特点及糖尿病肾病病理改变。

The study of type 2 diabetic rat model and nephropathy

Objective To study the feasibility of the high-sucrose-high-fat diet and injected with the low dose STZ induced the model of type 2 diabetic rat,and observed the characteristic of nephropathy.Methods SD rats fed with high-sucrose-high-fat diet for 8 weeks,and injected with a low dose of STZ(35mg/kg) into abdominal cavity to induce hyperglycemia.Determine serum glucose,fasting serum insulin,serum fat,serum creatinine,blood urine nitrogen,and kidney construction by optical microscope and electron microscope.Results The HSFG rats injected with a low dose STZ showed increase in body weight,blood glucose and fat in comparison with normal control group while ISI was reduced.At the 12 week,kidney weight/body weight,urine microalbumin,blood urea nitrogen,serum creatinine,mean glomerular area,mean mesangial matrix area,mean mesangial matrix area/mean glomerular area ratio were significantly increased as compared with normal control group,the capillary plexus area//mean glomerular area ratio was reduced.Conclusion The onset feature and kidney pathology of the diabetic rats induced by fed with high-sucrose-high-fat diet and injected with low dose STZ were somehow similar with Type 2 diabetes mellitus.The Type 2 diabetic rats model were successed.