药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究

目的建立简易的采用rDNA ITS区作为分子标记对中药石斛的基源植物进行分子鉴定的技术。方法 用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)方法获得ITS片段的限制性图谱。束花石斛及其形态相似种的PCR扩增产物用Cla I和Apa LI酶切,流苏石斛及其形态相似种的PCR扩增产物用Sph I酶切。结果根据预测的PCR-RFLP图谱,可以鉴别药用植物束花石斛、流苏石斛及它们的形态相似种。用这种方法鉴定了市场上收集的25件商品束花石斛、流苏石斛新鲜药材的原植物。结论rDNA ITS的PCR-RFLP可用于鉴定石斛药材的原植物,具有简便、费用低的优点。

Molecular identification of medicinal plants: Dendrobium chrysanthum, Dendrobium fimbriatum and their morphologically allied species by PCR-RFLP analyses

AIM: To establish a simple method for molecular identification of original plants of D. chrysanthum and D. fimbriatum using molecular marker rDNA ITS region. METHODS: Restriction patterns of ITS fragments were obtained using PCR-RFLP method. The PCR products of D. chrysanthum and its morphologically allied species were digested at 37 degrees C by Cla I and Apa LI, those of D. fimbriatum and its morphologically allied species were digested by Sph I. RESULTS: D. chrysanthum, D. fimbriatum and their morphologically allied species could be identified by predicted restriction profiles of PCR-RFLP. The botanical origin of twenty-five fresh samples of "Shihu" collected in markets was identified by this method. CONCLUSION: The results showed that PCR-RFLP analysis of the rDNA ITS region is a feasible, simple and inexpensive method for determining the botanical origin of the traditional Chinese medicine "Shihu".