膜联蛋白A1和增殖细胞核抗原在胰腺癌组织中的表达及其相互关系

目的 研究膜联蛋白A1(annexin A1,ANXA1)在胰腺癌中的表达及其与增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的相关性.方法 应用组织芯片制作仪构建芯片,芯片上有39例正常胰腺组织,42例胰腺癌组织,7例胰岛细胞瘤组织,8例壶腹腺癌组织.在连续的芯片切片上使用免疫组化法检测ANXA1和PCNA蛋白的表达,分析两者的相关性.结果 构建的组织芯片在免疫组化染色后的掉片率为11.7%～13.3%,满足实验要求.ANXA1在胰腺癌中的阳性表达率为71.4%(30/42),明显高于正常胰腺组织18.4%(7/38)的阳性率(P<0.01).PCNA在胰腺癌中的阳性表达率为73.8%(31/42),也明显高于正常胰腺组织22.2%(8/36)的阳性率(P<0.01).胰腺癌组织ANXA1表达与PCNA表达显著相关(P<0.01).结论 胰腺癌ANXA1蛋白的表达与PCNA表达同步增加.

Expression of annexin A1 and PCNA in pancreatic cancer and their correlation

Objective To investigate the expressions of the annexin A1 ( ANXA1 ) in pancreatic cancer and the correlations with proliferating cell nuclear antigen (PCNA). Methods Tissue microarray instrument was used to construct the chip. There were 39 samples of normal pancreas tissue, 42 samples of pancreatic cancer, 7 samples of islet cell tumor and 8 samples of ampullary carcinoma. The expressions of ANXA1 and PCNA protein were examined by immunohistochemistry and their correlation was analyzed. Results The off-chip rate of tissue chips after immunohistochemistry was 11.7% ～ 13.3%, which could satisfy the needs for experiment. Immunohistochemical results of pancreatic cancer tissue microarray showed that the positive rate of ANXA1 expression in pancreatic cancer was 71.4% (30/42), which was significantly higher when compared with that of normal pancreas tissue ( 18.4%, 7/38; P < 0. 01 ). The positive rate of PCNA expression in pancreatic cancer was 73.8% (31/42), which was significantly higher than that of normal pancreas tissue (22.2%, 8/36; P < 0.01 ). The expression of ANXA1 had a significant correlation with PCNA in pancreatic cancer ( P < 0.01 ). Conclusions The expression of ANXA1 protein was significantly increased in pancreatic cancer. The expression of ANXA1 was significantly correlated with the expression of PCNA.