RP-HPLC法测定甘草中甘草酸差向异构体的含量

目的建立RP-HPLC法同时测定甘草药材中甘草酸差向异构体的含量。方法采用HypersilC18(250 mm×4.6 mm,5μm)柱,以甲醇-体积分数为1%的醋酸溶液(体积比为56∶44)为流动相,流速为1.0 mL.min-1,检测波长为250 nm,柱温为35℃。结果α-甘草酸和β-甘草酸的线性范围分别为0.010~0.209 g.L-1(r=0.9995)和0.052~1.034 g.L-1(r=0.9996)。2种成分的平均加样回收率(n=9)分别为99.7%和99.4%。结论该方法可用于甘草药材中甘草酸差向异构体的同时含量测定。

Simultaneous determination of epimer of glycyrrhizic acid in Radix glycyrrhizae by RP-HPLC

Objective To develop an RP-HPLC for simultaneous determination of the epimers of glycyrrhizic acid in Radix Glycyrrhizae.Methods The separation was performed on a Hypersil C18 column(250 mm×4.6 mm,5 μm),with methanol-1% acetic acid(V∶V=56∶44) as mobile phase and the flow rate was at 1.0 mL·min-1.The detective wavelength was set at 250 nm and the column temperature was 35 ℃.Results The liner ranges of α-glycyrrhizic acid and β-glycyrrhizic acid were 0.01-0.20 g·L-1(r=0.999 5) and 0.05-1.00 g·L-1(r=0.999 6).The average recoveries(n=9) of the two compontents were 99.7% and 99.4%.Conclusions The method is simple,accurate and reproducible.It can be applied for the simultaneous determination of the epimers of glycyrrhizic acid of Radix Glycyrrhizae.