大黄通气口服液的提取纯化工艺优选

目的:优选大黄通气口服液的提取纯化工艺.方法:以挥发油提出量为指标,对木香、枳实等药材中挥发油提取工艺进行正交试验优化,考察因素为加水量、浸泡时间及提取时间；以水提取物得率和大黄酸、大黄素、大黄酚总量为考察指标,采用正交设计法考察煮提时间、煮提次数和加水量等因素对大黄通气口服液水提取工艺的影响；以干浸膏得率与大黄酸、大黄素、大黄酚总提取率为考察指标,采用正交试验法考察药液相对密度、乙醇体积分数及醇沉时间等因素对大黄通气口服液乙醇沉淀工艺的影响.结果:优选的挥发油提取工艺为加4倍量水蒸馏2h;最佳水提取工艺为加8倍量水煎煮2次,每次2h；优选醇沉工艺为浓缩至相对密度1.20(80℃),加入95％乙醇使药液含醇量为50％,醇沉时间为24 h.结论:该提取纯化工艺合理、可行,适合工业生产的需要.

Optimization of Extraction and Purification Technology for Dahuang Tongqi Oral Liquid

Objective:To optimize extraction and purification technology for Dahuang Tongqi oral liquid.Method:With volume of volatile oil as index,orthogonal test was used to optimize extraction technology of volatile oil from Aucklandia lappa,Citrus sinensis and so on with soaking time,extraction times,the amount of water as factors;With yield of water extract,total content of rhein,emodin and chrysophanol as indexes,orthogonal test was used to investigate factors of water extraction process for Dahuang Tongqi oral liquid,such as extraction time,extraction times and the amount of water;With yield of dry extract,total extraction ratio of rhein,emodin and chrysophanol as indexes,orthogonal test was used to investigate factors of ethanol precipitation process for Dahuang Tongqi oral liquid,such as relative density of liquid,the concentration of ethanol and alcohol precipitation time.Result:Optimized extraction technology of volatile oil was:distilling 2 h with 4 times the amount of water;optimum water extraction technology was:boiling 2 h with 8 times the amount of water,2 h per time;optimized alcohol precipitation process was:concetrated to relative density of 1.20(80 ℃),95% ethanol was added to liquid contained ethanol of 50%,alcohol precipitation time 24 h.Conclusion:Optimized extraction and purification technology for Dahuang Tongqi oral liquid was reasonable and feasible,it was suitable for requirements of industrial production.