甘露聚糖酶产生菌F1-5的鉴定

目的]鉴定1株高产甘露聚糖酶菌株。方法]以高产甘露聚糖酶的菌株为对象，采用形态观察、生理生化试验及16S rDNA系统发育分析手段对其进行鉴定。结果]该菌株在牛肉膏蛋白培养基上培养24h后，菌落表面粗糙，不透明白色，革兰氏阳性，杆状，能形成芽孢，表明其属于芽孢杆菌属。对F1-5进行生理生化特征鉴定，结果表明，其为枯草芽孢杆菌或蜡样芽孢杆菌。PCR扩增获取该菌的16S rDNA基因。经BLAST同源序列比对，结果显示，菌株F1-516SrDNA与枯草芽孢杆菌的16S rDNA具有99％的同源性，表明F1-5为枯草芽孢杆菌。结论]该研究为甘露聚糖酶工业化开发应用奠定了基础。

Identification of Mannanase-producing Bacterial Strain F1-5

Objective] The aim was to identify a high-yield strain of mannanase.Method] With high-yield strain of mannanase as theobject,it was identified by morphological observation,physiological and biochemical experiment and 16S rDNA phylogenetic analysis.Result] After the strain was cultured for 24 h in the beef-protein medium,the surface of the colony was rough,the bacteria was opacity,white,gram-positive,rod and it could form spore,which showed that it belonged to bacillus.The identification on the physiological and biochemical characteristics of F1-5 showed that it was Bacillus subtilis or Bacillus cereus.The 16S rDNA gene of the bacteria was obtained by PCR amplification.The BLAST homology sequence alignment result showed that the 16S rDNA gene of the F1-5 had 99% homology with that of the B.subtilis,which showed that F1-5 was B.subtilis.Conclusion] The research laid the foundation for the industrialization development and application of mannanase.