黄连解毒汤对小鼠酒精性脂肪肝的预防作用

目的 研究黄连解毒汤(HLJDT)对小鼠酒精性肝损伤的预防作用,为黄连解毒汤临床防治酒精性肝病提供实验依据。方法 选取60只C57小鼠随机分为空白对照组、模型对照组和HLJDT低、中、高剂量(0.61、1.22、2.44 g/kg/d)模型组,每组各12只。模型组用50%乙醇灌胃,同时采用HLJDT进行实验干预,6周后,对各组小鼠的体质量、肝指数、血清生化指标以及肝脏和结肠的病理学改变进行综合对比分析。结果 HLJDT各剂量模型组小鼠的体重增长量均显著增加(P<0.05),肝指数明显降低(P<0.05),同时HLJDT各剂量模型组能显著降低血清ALT、AST和TG的含量(P<0.05)。病理结果显示HLJDT各剂量均对酒精性肝损伤有显著的改善作用,且从低剂量到高剂量组对肠粘膜通透性具有递增的改善作用。结论 黄连解毒汤可显著降低肠粘膜的通透性,对小鼠酒精性肝损伤具有一定的预防保护作用。

Prevention effects of Huanglian Jiedu Decoction on alcoholic fatty liver in mice

Objective To study the preventive effect of a Chinese traditional medicine, Huanglian Jiedu Decoction (HLJDT), on alcoholic fatty liver in mice, and provide experimental evidence for clinical prevention and treatment of alcoholic liver disease. Methods Sixty healthy SPF male C57 mice were randomly divided into control group, model group and low, medium and high dose HLJDT(0.61, 1.22, 2.44 g/kg/d)groups, 12 mice in each group. The mice of model groups were given gastric gavage of 50% ethanol, combined with concomitant HLJDT intervention for 6 weeks. The mice of control group received the same volume of saline. The body weight, liver index, serum biochemical indexes were determined and histopathological changes in the liver and colon tissues were examined and analyzed at 6 weeks after the experiment. Results Body weight gains of the mice in all the three dose HLJDT groups were significantly higher than that of the control group (P<0.05),liver index and the serum ALT, AST and TG contents were significantly decreased (P<0.05 for all). Histological changes in the liver of all the three dose HLJDT groups were significantly milder than those of the model control group, and also improving of the colon mucosal alterations was observed in the three dose HLJDT groups in a dose-dependent manner. Conclusions Huanglian Jiedu Decoction can well reduce the permeability of intestinal mucosa, and exerts certain preventive and protective effects on alcoholic liver injury in mice.