| 改性硅藻土对脂肪酶固定化研究 |
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| 引用本文： | 刘媛媛,郑永杰,田景芝.改性硅藻土对脂肪酶固定化研究[J].化学工程师,2014(3):14-17. |
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| 作者姓名： | 刘媛媛 郑永杰 田景芝 |
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| 作者单位： | 齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006 |
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| 基金项目： | 齐齐哈尔大学青年教师科研启动支持计划资助（2010K-M24）;黑龙江省教育厅资助项目（12521612） |
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| 摘 要： | 以改性硅藻土为载体,采用吸附法对脂肪酶进行固定化。对固定化条件进行优化。得到较佳的固定化条件:固定化时间为4h;温度为40℃;pH值为7.5;固定化酶在40℃下保温2h后酶活损失很小,固定化酶重复性达到最佳状态。
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| 关 键 词： | 改性硅藻土 固定化 脂肪酶 |
Study on modified diatomite for lipase immobilization |
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| LIU Yuan-yuan,ZHENG Yong-jie,TIAN Jing-zhi.Study on modified diatomite for lipase immobilization[J].Chemical Engineer,2014(3):14-17. |
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| Authors: | LIU Yuan-yuan ZHENG Yong-jie TIAN Jing-zhi |
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| Affiliation: | ( College of Chemistry and Chemistry Engineering, Qiqihaer University, Qiqihaer 161006, China) |
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| Abstract: | The lipase was immobilized by adsorption method with modified diatomite as carrier. The optimized conditions were immobilized time is 4h, temperature is 40℃, pH value is 7.5. The results showed that enzyme activity loss was little when under 40℃ and heat preserved 2h. The immobilized enzyme repeatability achieve the best. |
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| Keywords: | modified diatomite immobilization lipase |
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