脐血CD34~+细胞体外短期培养扩增研究

为寻找更有效的体外扩增脐血CD34 + 细胞的造血细胞因子组合 ,采集健康产妇脐带血 ,用免疫磁珠法分选CD34 + 细胞。采用SCF、FLT3 L、TPO和IL 34种具有早期作用的细胞因子的不同组合进行脐血CD34 + 细胞短期无血清液体培养 ,观察培养前后有核细胞、CD34 + 细胞、CD34 + /CD38- 细胞、CFU GEMM、CFU GM和BFU E数量的变化。结果在 3种不同的细胞因子组合中 ,同时应用SCF、FLT3 L、TPO和IL 34种细胞因子培养 7d的扩增效果最好。突出的发现是在这种条件下CD34 + /CD38- 细胞亚群达到平均 1 97.9倍的扩增效果。提示 :SCF、FLT3 L、TPO和IL 34种细胞因子是脐血CD34 + 细胞体外扩增理想的细胞因子组合

Study on Cord Blood CD34+ Cell in Short-term Culture in Vitro Amplification

The objective was to explore the optimal cytokine combinations for in vitro amplification of the cord blood CD34 cell. We collected the cord blood from normal full-term deliveries, and isolated CD34 cells using mini MACS laboratory separation system. The purified CD34 cells were cultured in static short-term serum free culture system with different combinations of SCF, FLT3-L, TPO and IL-3. Result: Within the three different cytokine combinations, the combination of SCF, FLT3-L, TPO and IL-3 was proved to be the most effective, and the notable result was that the amplification of CD34 /CD38 - subsets reached 197.9 folds. Conclusion: The SCF, FLT3-L, TPO and IL-3 combination is the optimal cytokine combination for in vitro cord blood CD34 cell amplification.