Incorporation of 1,2-14C-ethanolamine into subfractions of rat liver phosphatidylethanolamines and phosphatidylcholines |
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Authors: | J Tinoco G Sheehan S Hopkins R L Lyman |
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Affiliation: | (1) Department of Nutritional Sciences, University of California, 94720 Berkeley, California |
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Abstract: | The incorporation of 1,2-14C-ethanolamine into the liver phosphatidylethanolamines (PE) and phosphatidylcholines (PC) in female rats was studied. These
phosphatides were fractionated according to their degrees of unsaturation and the specific activities of monoenoic, dienoic,
tetraenoic and hexaenoic fractions were measured at intervals of 1, 20, 60 and 300 min after injection of the labeled precursor.
Hexaenoic and dienoic PE incorporated and lost the label rapidly. Although the labeled precursor was incorporated into tetraene
PE at a similar rate, this fraction attained a relatively low specific activity that remained essentially constant between
10 and 300 min after injection of the label. Hexaenoic PC had the highest specific activity among the PC fractions at all
time periods. Estimations of the rate of loss of radioactivity in the hexaenoic PE fraction and its appearance in hexaenoic
PC indicate that the docosahexaenoic acid is conserved, possibly by being reincorporated into PE after becoming a part of
the hexaenoic PC species. The high rate of turnover of the hexaenoic PE also suggests that this fraction might have some special
role in endogenous choline synthesis. |
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