运动性血清酶升高与骨骼肌自由基代谢变化相关性分析

大鼠进行一次性力竭运动至力竭后 ,实验结果表明 :力竭运动使大鼠股四头肌红肌自由基代谢水平显著升高 ,使股四头肌红肌生物膜结构完整性丧失及正常机能的损害 ,最终导致细胞膜通透性增加 ,肌肉细胞内酶大量外流 ,造成血清酶升高 ,运动性血清酶升高与骨骼肌自由基代谢具有高度相关性

An Analysis of Correlation between Serum Enzyme Increasing with Free Radical Metabolism Variation Cased by Exercise

The superoxide dismutase (SOD), glutathion peroxidase (GSH Px) and alkaline phosphatase (AKP) activity in red muscle of quadriceps femoris and creatine kinase (CK) activity in red serum were tested after exhaustive exercise. SOD activity in red muscle homogenate increased, but no significant rising occurred after exhaustive exercise in experiment group. MDA content in red muscle homogenate increased significantly and GSH Px activity decreased obviously in experiment group. CK in serum and AKP activity in red muscle homogenate increased significantly. The rising level of MDA in red muscle homogenate was significantly correlated with the rising of CK in serum. The result indicated that exhaustive exercise caused significant increase of free radical activity in red muscle, which resulted in the damage of the normal function of cell membrane, the extensive injury of the structure of cell membrane and caused increase permeability of cell membrane. Therefor, a great deal of CK in cell flew off, and as a result, the CK activity in serum increased. The rising serum enzyme was significantly correlated with muscle free radical metabolism change.