双PI3K/mTOR抑制剂NVPBEZ235对宫颈癌Hela细胞的放射增敏作用

目的: 探讨双PI3K/mTOR 抑制剂 NVPBEZ235对宫颈癌Hela细胞的放射增敏作用。方法: 四甲基偶氮唑盐（MTT）及集落克隆法测定各因素对Hela细胞的抑制作用。通过流式细胞仪检测药物联合射线（irradiation，IR）对宫颈癌细胞周期的影响。结果: 与对照组相比，NVPBEZ235在体外能够抑制人宫颈癌细胞的生长。NVPBEZ235、IR联合作用细胞，在体外可以使细胞周期阻滞于G2/M期，二者具有协同作用，联合作用效果显著大于单药作用。结论: 20% IC50 NVPBEZ235在体外能够促进细胞对射线照射的敏感性，抑制细胞的增殖从而实现对宫颈癌细胞的放射增敏作用。

Radio sensitizing effect of double PI3K/mTOR inhibitor NVPBEZ235 on Hela cells of cervical cancer

AIM: To investigate the effect that external irradiation of dual PI3K/mTOR inhibitors NVPBEZ235 on cervical cancer Hela cells. METHODS: Cervical cancer Hela cells treated with different concentrations of NVPBEZ235 and the radiotherapy (irradiation, IR) were examined for cell viability using MTT assay and colony forming ability. The cycle arrest of cervical cancer cells combined drug with IR was analyzed by using the flow cytometry. RESULTS: Compared with the control group, NVPBEZ235 can inhibit human cervical carcinoma cell in vitro (P<0.05). The combined use of NVPBEZ235 significantly enhanced the inhibitory effect of IR (P<0.05) in Hela cells. In vitro, NVPBEZ235 and IR, which had synergistic effect, can block cell cycle to G2/M period by the combined effect which was significantly greater than the effect of one single drug (P<0.05). CONCLUSION: 20% IC50 NVPBEZ235 can promote the cell's radiation sensitivity, inhibit the proliferation of cells, and thereby, increase its radio sensitivity to cervical cancer cells.