脱氧核酶抑制耐甲氧西林金黄色葡萄球菌耐药基因的表达

目的 探讨抑制耐甲氧西林金黄色葡萄球菌(MRSA)耐药基因blaR1 表达的脱氧核酶对MRSA耐药性的影响。 方法 以耐药基因blaR1 的mRNA为靶点, 设计合成脱氧核酶DrzB, 导入细菌后通过平板克隆形成实验观察DrzB 对MRSA 细菌耐药性的影响, 通过RT-PCR 观察DrzB 对耐药基因blaR1表达的抑制作用。 结果 加入DrzB 后培养的MRSA,在含苯唑西林(6 mg·L^-1)的M-H 琼脂培养基表面生长的菌落单元计数低于对照组(P <0.01), blaR1的mRNA 表达水平也较对照组降低(P <0.01)。 结论 导入以blaR1 的mRNA 为靶基因的DrzB 阻断耐药基因表达, 可以部分恢复MRSA 的抗生素敏感性。脱氧核酶DrzB 是一种特异性的、有效的基因治疗剂。

DNAzymes inhibit the expression of Methecillin-resistant staphlococcus aureus drug-resistant genes

AIM: To evaluate the inhibition effects of DNAzymes specific to Methecillin-resistant staphylococcus aureus (MRSA)drug-resistant gene blaR1 on the expressions of drug-resistance. METHODS: mRNA of blaR1 was chosen as a target gene and DNAzyme DrzB specific to it was designed and synthesized.After DrzB was introduced into the MRSA, drug-resistant characters of MRSA were evaluated by cloning efficiency.The inhibition effects of DrzB on the expressions of drug-resistant gene blaR1 were observed by RT-PCR in MRSA. RESULTS: Colony forming units (CFU)of MRSA incubated with DrzB on the M-H agar added oxa (6 mg·L^-1)were less than those of control group (P <0.01).Levels of blaR 1 mRNA of the DrzB group were lower than those of the control group (P <0.01). CONCLUSION: Antibiotic sensitivity on MRSA can be partially restored by exogenously delivered DrzB targeted to mRNA of blaR1 in blocking drug-resistant genes expression.DNAzyme DrzB is proved a specific and effective gene therapeutic means.